Learn how to phenotype LSK, LSK/SLAM, and ESLAM hematopoietic stem and progenitor cells from mouse bone marrow using flow cytometry.

LSK cells with expression of CD150 and no expression of CD486 HSCs are a rare cell type in mouse bone marrow, with a frequency of about 0.002%7. At this low percentage, a large …

2022年2月10日 · 来自辛辛那提儿童医院医学中心的Damien Reynaud博士结合多种经典的造血系统表型分析方案，开发了可同时分析小鼠HSC及HSPC亚群的14色全光谱流式分析方案，旨在 …

首先以FSC-A 和SSC-A为轴,画出主要细胞群,然后以FSC-A 和FSC-H 去除粘连细胞,画出单个细胞群,再画出Lin-细胞群,一般占单个细胞群的10% ,然后画出Sca1+cKit+细胞群 (LSK, Lin …

Flow cytometry has been critical in establishing methods to isolate and identify hematopoietic stem cells and their progenitors. For more than 30 years, researchers have been uncovering …

2012年6月26日 · In this article, we have reviewed some of the common methods for HSC purification by flow cytometry, discussed analysis of hematopoietic progenitors and peripheral …

开始的时候依据光散射设门精确圈出原始细胞（Primitive cells），然后排除结合的细胞团和死细胞。 首先排除Lin+细胞，接着设门圈出Sca1+ c-Kit+ 细胞群，此群细胞即为LSK细胞。 各群细 …

2024年8月12日 · In this study, we assessed the transcriptomic signature of HSCs in db/db mice, a well-known and widely used model for T2D. LSK cells of db/db mice enriched using a cell …

2024年7月1日 · Flow cytometry demonstrated substantial recovery in the proportions of LSKs, Cd34 − LSK, HSCs, and progenitor cells (Figures 6 D–6H). Additionally, Ppif …

2021年6月1日 · Flow cytometry has been widely used to detect a single event by means of multiparametric fluorescence measurements. Here we describe a method to analyze subsets …