Learn how to phenotype LSK, LSK/SLAM, and ESLAM hematopoietic stem and progenitor cells from mouse bone marrow using flow cytometry.

cell population is also termed LSK. Less than 10% of LSK cells are long-term HSCs (LT-HSCs).3 Two main strategies are commonly used to identify mouse LT- HSCs within the LSK cell …

摘要:造血干细胞 (Hematopoietic Stem Cells, HSCs) 是具有长期自我更新,以及分化为各类成熟血细胞能力的成体干细胞。 本文以小鼠骨髓组织为例,使用7 色共14种抗体,标记出在骨髓细胞中的 …

The hematopoietic progenitor population was initially refined using a light scatter gate followed by discrimination of doublets and dead cells. Lin+ cells were excluded, followed by gating on the …

2012年6月26日 · Regarding where to draw the SP gate, particularly how far towards the top of the SP, we prefer a conservative SP gate, while attempting to maximize cell number yield and …

2018年7月24日 · Importantly, 24 h post stimulation, the increase in Sca1 shifted almost the entire Lineage - cKit + Sca1 − (LK) population into the Lineage − cKit + Sca1 + (LSK) gate (Fig. 1a, …

2008年11月20日 · Multipotent long-term HSCs (LT-HSCs) reside in the bone marrow and through a process of asymmetric cell division, can self-renew to sustain the stem cell pool or …

... a plot of PECy7, set CD150 + gate under Sca-1 + c-Kit + gate (Gate 2) to include clearly separated, the most positive population. Normally 20%-30% of CD48 -LSK cells are expected …

LSK细胞就是小鼠骨髓中的造血干细胞。 从小鼠后退骨中提取出细胞，然后染色，上流式细胞仪，最后Gate出lsk细胞就认为是造血干细胞。

2022年10月19日 · One of the biggest challenges in HSC analysis postirradiation is HSC gating by flow cytometry. Widely established gating strategies for HSCs from mouse bone marrow begin …