在骨髓分支中，CMPs 产生了 2 个更多的受限祖细胞，即巨核细胞-红细胞祖细胞 (MEPs) 和粒细胞-巨噬细胞祖细胞 (GMPs)，其中MEPs 产生了红细胞祖细胞和单能巨核细胞前体。 图 1A）。 8、9、28. 尽管经典的造血模型在其谱系潜力的二元概念中很简单，但这种造血模型已受到新祖种群鉴 …

2023年12月1日 · In HSC-enriched LSK cells, MTCH2 limits the expression of the mitochondrial respiratory chain complex to maintain low-OXPHOS levels and low MMP [33]. In addition, MTCH2 maintains balanced NAD/NADH levels for optimum redox control to limit mitochondrial ROS (reactive oxygen species, mitochondrial volume and thus prevents abnormal LSK proliferation.

2021年8月2日 · Limited knowledge of cellular and molecular mechanisms underlying hematopoietic stem cell and multipotent progenitor (HSC/MPP) expansion within their native niche has impeded the application of...

2021年2月4日 · We show that mitochondrial membrane potential (MMP) can be used to prospectively isolate chronologically old HSCs with transcriptional features and functional attributes characteristic of young HSCs, including a high rate of transcription and balanced lineage-affiliated programs.

2017年9月12日 · Moreover, LSK cells, which include both HSCs and MPPs (together known as HSPCs), showed the highest expression among BM cells (Fig. 1c). In the myeloid lineage, LSK cells differentiate into...

2007年6月22日 · Because the LSK population contains all multipotent precursors including LT-HSCs, ST-HSCs, and MPPs, lineage-restricted progenitor populations were first sought outside the LSK fraction. A series of efforts focused on the isolation of lineage-committed progenitors resulted in the successful isolation of the CLP ( Kondo et al., 1997 ).

2021年6月10日 · Hematopoietic stem cells (HSCs) and distinct multipotent progenitor (MPP) populations (MPP1-4) contained within the Lin-Sca-1+c-Kit+ (LSK) compartment have previously been identified using diverse surface-marker panels. Here, we phenotypically define and functionally characterize MPP5 (LSK CD34+CD13 …

2024年7月1日 · Flow cytometry demonstrated substantial recovery in the proportions of LSKs, Cd34 − LSK, HSCs, and progenitor cells (Figures 6D–6H). Additionally, Ppif haploinsufficiency reduced ROS levels and inhibited mPTP activation, significantly restoring MMP in the double knockout HSCs (Figures 6I–6Q).

2007年10月15日 · The CD34 + LSK population, termed as the MPP, is composed of multiple progenitor populations. The LMPP is the MPP population expressing Flt3, and is largely committed to the myelo-lymphoid...

2021年4月20日 · 本研究表明，线粒体膜电位（MMP）可用于前瞻性分离老年的HSC和具有转录特征和功能属性的年轻HSC。 MMP是比实足年龄这个因素更强能决定HSC定量和定性转录状态因素，在其天然微环境内操纵HSC中MMP的转录后果表明存在因果关系。 因此，研究表明，老年HSCs中MMP的药理作用增强可增加移植后的植入潜能，并在稳定状态下逆转由骨髓偏向的外周血输出。 本研究证明MMP是老HSC异质性的来源，它的药理学操作可以改变转录程序，对HSC …