【单细胞+空间转录组应用案例】单细胞时空转录组学鉴定胎儿肝 …
2021年9月3日 · e轨迹分析重建hsc/mpp 到淋巴和骨髓祖细胞的谱系分化。f热图显示了三种 hsc/mpp 亚型中hsc/mpp 特征基因和cd93的表达模式。g使用 cd93 + slam-lsk (cd93 + hsc) 和 cd93 – hsc进行初次移植后 8 周受者外周血 (pb) 中供体来源的嵌合体比例。
Kinase-inactivated CDK6 preserves the long-term ... - ScienceDirect
2024年7月11日 · Uniform manifold approximation and projection (UMAP) visualization of 11 LSK cell clusters (bottom). Colors indicate different clusters.
External signals regulate continuous transcriptional states in
2021年12月23日 · We performed single-cell RNA sequencing (scRNA-Seq) on functionally validated mouse HSCs and LSK (Lin-, c-Kit+, Sca1+) progenitors after in vivo pharmacological perturbation of niche signals interferon, granulocyte colony …
Hematopoiesis under telomere attrition at the single-cell ... - Nature
2021年11月25日 · As the results obtained using the LSK populations’ surface markers aligned with our unbiased scRNA-seq analysis of LSK cells, we isolated MPP2, MPP3, and MPP4 cells and assessed how telomere ...
Interleukin-1β modulates lymphoid differentiation of
2024年11月26日 · Log fold change of differentially expressed genes (DEGs) between one-week transplanted EGFP + EYFP + (GY) cells (BMT) and the steady-state LSK cells (LSK) from the three G + MPP groups.
Implications of stress-induced gene expression for ... - Nature
2024年1月16日 · Using uniform manifold approximation and projection (UMAP), we distinguished cell clusters based on their treatment (Fig. 2e). Cells kept on ice formed a cluster regardless of TP treatment, while...
Regulation of lymphoid-myeloid lineage bias through regnase-1/3 ...
2024年1月18日 · Reg1 and Reg3 are ribonucleases critical for determining lymphoid fate and restricting myeloid differentiation in HSPCs. Reg1/Reg3-mediated decay of Nfkbiz, crucial for HSPC lineage choice, is potently manipulated by an antisense oligonucleotide.
Single-cell epigenetic landscape of heme-trained HSPCs in mice.
The projection of the TF motif deviation scores onto the Uniform Manifold Approximation and Projection (UMAP) plots visualizes the notable shifts in the abundance and cell-specific occurrences of...
PERK reprograms hematopoietic progenitor cells to direct tumor ...
Our results indicate that PERK-mediated HSPC preconditioning is a previously unappreciated early event and a crucial regulatory mechanism of MDSC generation. Targeting this signaling in the spleen may provide novel therapeutic opportunities to selectively redirect tumor-promoting myeloid responses at the source.
Molecular analysis of LSK cells uncovers cell cycle status...
Download scientific diagram | Molecular analysis of LSK cells uncovers cell cycle status heterogeneity in MPPs. (A) UMAP projection for CITE-seq–identified clusters and marker genes from the ...