Nov 5, 2024 · A pool of human long-term hematopoietic stem cells (LT-HSC) maintains life-long hematopoiesis by generating shorter-living progenitors that ultimately replenish mature cells.

Jun 14, 2019 · LT-HSCs are a rare, quiescent population in bone marrow and have full long-term (> 3~4 months) reconstitution capacity, whereas ST-HSCs only have a short-term (mostly < 1 month) reconstitution ability.

Mar 12, 2024 · Utilizing the Hoxb5 reporter system to prospectively isolate long-term HSCs (LT-HSCs) and short-term HSCs (ST-HSCs), we found that young and aged LT-HSCs co-transplanted into the same recipients demonstrated nearly equivalent myeloid lineage output, contrary to the theory of myeloid-biased HSC clones.

Nov 22, 2022 · LT-HSCs are self-renewing cells that exist in a predominantly quiescent/dormant state, while ST-HSCs (also known as progenitor cells) are ready to differentiate but can also return to dormancy. Although LT-HSCs are well characterized, ST-HSCs are more heterogeneous and lack widely used standardized surface markers, particularly in humans.

Nov 15, 2022 · Long-term hematopoietic stem cells (LT-HSC) are responsible for life-long blood production and show functional erosion due to aging or inflammatory dysregulation. However, the molecular programs underlying aging or LT-HSC responsiveness to emergency hematopoiesis as triggered by stress stimuli, notably inflammation, are poorly understood.

Nov 2, 2023 · Simple combinations of markers that reliably demarcate human long-term repopulating hematopoietic stem cells (LT-HSCs) would facilitate our understanding of their unique molecular state and the development of stem cell-based therapeutics.

Apr 19, 2023 · To gain more functional human HSCs ex vivo, Sakurai et al. established a recombinant-cytokine-free and albumin-free culture system, i.e. PCL-PVAc-PEG-based culture, in combination with 740Y-P, butyzamide and UM171, to improve the long-term expansion of human cord blood HSCs.

Apr 11, 2023 · LT-HSCs are at the top of the hematopoietic cascade, and they can maintain multi-lineage hematopoietic reconstruction for a long period; notably, they are the only cell population with true HSC characteristics. The ST HSCs and progenitor cells can only maintain normal hematopoiesis for 6–8 weeks .

Sep 1, 2023 · In the definitive hematopoietic wave, also called HSC-dependent hematopoiesis, the long-term HSCs (LT-HSCs) are generated from the hemogenic endothelium (HE) of aorta-gonad mesonephros (AGM) region through endothelial-to-hematopoietic transition (EHT) (murine E10.5, human 5–6 weeks), as well as in the placental labyrinth, vitelline and ...

Here we describe an LT-HSCs methylcellulose colony-forming assay, as a fast complementary in vitro method to directly assess LT-HSC differentiation capacity. As described in Kerenyi et al. (2013), this technique acts as a powerful tool to differentiate between LT-HSC or progenitor cell differentiation defects.