What Does This Nomenclature Mean? The LysMcre knock-in/knock-out allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements.

2022年1月6日 · By crossing septin7 -floxed mice with Lyz2 -Cre mice carrying the Cre recombinase inserted in the Lysozyme-M (Lyz2) gene locus we aimed the specific deletion of septin7 in myeloid cells, such as monocytes, macrophages and granulocytes. Septin7 flox/flox : Lyz2- Cre mice show no alterations in the myeloid compartment.

JAX uses a very high speed Taq (~1000 bp/sec), use cycling times recommended for your reagents. JAX uses a 'touchdown' cycling protocol and therefore has not calculated the optimal annealing temperature for each set of primers.

2022年1月5日 · In the present study, we crossed the Septin7flox mice with the myeloid-specific Lyz2 -Cre line (Clausen et al., 1999) to generate a tissue-specific Septin7- knockout (KO) mice and to investigate the role of septins in myeloid cells.

2022年12月5日 · Bmal1LoxP/LoxP were crossed with Lyz2Cre mice, which express Cre recombinase under the control of the Lyz2 promoter to produce progeny that have Bmal1 excised in the myeloid lineage.

2025年1月2日 · The LysMcre knock-in/knock-out allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene (Lyz2); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements.

2025年2月28日 · Our study examined Lyz2Cre effects independently, unlike most research pooling floxed and Cre control mice. Results indicated Lyz2Cre expression alone impacts glucose metabolism, challenging the notion that Cre expression is harmless.

2021年5月14日 · Lyz2Cre + HBEGF-/-mice are protected from bleomycin-induced fibrosis. HBEGF -/- mice have decreased amounts of hydroxyproline, decreased levels of collagen 1, collagen 3, and fibronectin mRNA transcript, lessened interstitial thickening, and increased day 21 survival compared to Lyz2Cre + animals.

The above short name is used in most MPD data displays for conciseness. No studies involving this strain are currently available in MPD.

2024年12月17日 · View Lyz2<tm1 (cre)Ifo> allele: origin, molecular description and human disease models, images, gene associations, and references.