Investigation of hepatobiliary or bone disease. Increased levels in liver disease (particularly in association with cholestasis), bone disease (with increased osteoblastic activity eg, Paget's disease), some bony metastases (especially prostate and breast), and at times in malignancy without liver or bone metastases (Regan isoenzyme).

2015年6月15日 · Herein, we fabricated a kind of bi-MBs by co-immobilizing antibody and ALP signal molecules on magnetic nanobead surface. The bi-MBs were used not only as separation and enrichment carriers but also as signal carriers, which greatly enhanced target capture efficiency, simplified immunoreaction and improved detection sensitivity.

2016年4月15日 · Vancomycin, which has a strong antibacterial effect to Gram-positive bacteria, was adopted as one molecular recognition agent for bacterial detection. Magnetic beads (MBs) were functionalized with this antibiotic to effectively …

2017年4月15日 · By taking advantage of MBs and Zn 2+-stimulated dephosphorylation process of ALP, a dual signal amplification strategy was designed to construct a sensitive and direct plasmonic ELISA. To verify the dual signal amplification, RSV was chosen as a model pathogen for the plasmonic ELISA.

2015年11月21日 · 万古霉素对革兰氏阳性细菌具有很强的抗菌作用，被用作一种用于细菌检测的分子识别剂。 用这种抗生素对磁珠（MBs）进行功能化，以有效地浓缩金黄色葡萄球菌（S. aureus）。 此外，基于兔IgG Fc区与S细胞壁中蛋白A的结合，使用碱性磷酸酶（ALP）标记的兔免疫球蛋白G（ALP-IgG）作为第二种识别剂，可提高特异性。 金黄色的。 MBs浓缩的万古霉素/ S夹心复合物。 用一步温育方案形成金黄色/ ALP-IgG。 然后通过注入底物溶液来定量金黄色 …

2019年11月30日 · In this assay, MBs were employed as carrier to load large amount of ALP molecules for signal amplification. The introduction of Zn 2+ to the detection system induced the accelerated dephosphorylation reaction of ALP to trigger the dispersion of aggregated AuNPs, resulting in the amplification of the signal (Fig. 15 b ).

2024年7月1日 · 亚稳态结合位点 (MBS) 已在多种分子动力学模拟中观察到，可被视为低亲和力变构结合位点 (ABS)，当配体移向正构结合位点 (OBS) 时，其充当踏脚石。 在此，我们证明MBS可以在配体设计中用作ABS，从而产生具有改善的结合动力学的配体。 通过将 ( S )-阿普洛尔 ( ( S )-ALP) 分子对接至与拮抗剂 ALP 结合的 β 2肾上腺素能受体 (β 2 AR) 的共晶结构中，设计了四种同二价双位配体 ( 1 – 4)。 配体4显示出与 ( S )-ALP 相似的效力和亲和力，但停留时间增加了 4 …

2020年9月21日 · Alkaline phosphatase-oligonucleotide-functionalized magnetic beads (MBs-DNA-ALP) were designed as probes to monitor the concentration of S. aureus. When S. aureus was present, a naturally secreted nuclease, micrococcal nuclease, cut the AT-rich DNA sequences of MBs-DNA-ALP into fragments, resulting in …

Electrophoresis of serum or other body fluid to demonstrate: (a) the isoenzymes of lactate dehydrogenase; or (b) the isoenzymes of alkaline phosphatase;

2021年8月3日 · When ALP is present, it catalyzes 3'-terminal dephosphorylation of poly-A MBs to induce TdT-mediated template-free polymerization, generating long chains of polythymidine (T) sequences. The long poly-T chains can function as the anchoring templates to hybridize with many poly-A MBs, leading to the unfolding of loop structures and the ...