The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures.

MCF10A cells exhibit a unique differentiated phenotype in 3D culture which may not exist or be rare in normal human breast tissue. Our results raise a question as to whether the commonly used MCF10A cell line is a suitable model for human mammary cell studies.

MCF 10A cell lines are immortalized, non-tumorigenic epithelial cells derived from human breast tissue. They are extensively used as in vitro models to study breast tumor progression due to their close mimicry of normal breast epithelium and their ability to undergo oncogenic transformation.

MCF-10A cells are commonly recognized as a ‘‘normal’’ breast epithelial cell line, the karyotype is stable and near-diploid, these cells are nonetheless cytogenetically abnormal. These cells harbor a deletion of the locus containing p16 and p14ARF, as well as amplification of MYC. Notably, MCF-10A cells express wild-type p53.

4 天之前 · MCF 10A cells are a widely used, non-tumorigenic breast epithelial cell line that serves as a valuable model in cancer research and cellular biology. Their genetic stability and ability to mimic key aspects of normal breast tissue make them instrumental for studying cell behavior, signaling pathways, and drug responses.

As a non-tumorigenic epithelial cell line derived from benign proliferative breast tissue, MCF10A cells are instrumental in mammary cell studies, offering insights into breast tumor progression and the dynamics of tumor cells in mammospheres.

2022年10月7日 · To develop a detailed understanding of the linkage between molecular and phenotypic changes, we generated a comprehensive dataset that catalogs the transcriptional, proteomic, epigenomic and...

The MCF 10A ECAD-EmGFP reporter cell line provides a convenient and sensitive platform for research on the mechanisms of metastasis in vitro and the development of new antitumor drugs for metastatic breast cancer.

The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures.

The human normal breast cell line MCF-10A is being widely used as a model in toxicity studies due to its structural similarity to the normal human mammary epithelium.