2000年3月1日 · Cultures with αCD3 had a rapid, large, and persistent decline in surCD3; the cCD3 median fluorescent intensity (MFI) declined gradually, over the entire culture period. With αCD3, surCD4 MFI and cCD4 MFI declined by days 4 to 8 (31% of ex vivo value, p < 0.001 and 47%, p = 0.033), as did surCD8 MFI (58%, p = 0.010).

2023年12月14日 · In this study, we present the development of truncated TCRα and TCRβ chains, termed mini-TCRs, which lack variable domains responsible for recognizing human leukocyte antigen (HLA)-peptide complexes. We successfully induced cytotoxic T lymphocytes (CTLs) from iPSCs by employing mini-TCRs.

2021年9月9日 · 下图中，细胞样品染CD3 FITC抗体为例，通过横坐标的荧光强度，把此细胞样品分为两群，左边一群是CD3 negative，右边一群是CD3 positive，进而我们可以知道此细胞样品中CD3阳性细胞的比例是多少。

2024年8月2日 · In this study, we assess the median fluorescent intensity (MFI) of CD4 and CD3 on cases of MF/SS and control samples to better define the intensities of CD4 and/or CD3 that are different from normal versus overlapping with normal in order to improve diagnostic accuracy. This study was approved by the Stanford University Institutional Review Board.

Cell proliferation index was defined as the inverse of the median fluorescence intensity (MFI) of the gated proliferated population times 100.

The results of flow cytometry experiments are presented as the percentages of CD3+ or CD3 + CD56+ cells revealing the expression of the studied protein (% of positive cells) and the relative expression, i.e. mean fluorescence intensity (MFI) measured in the samples.

Collectively, these studies indicate that relative CD3 MFI is a more reliable predictor of functional avidity than relative CD8 MFI, and that the predictive power is not improved by adding CD8 MFI. CD3 and CD8 expression identify high-avidity cells with superior cytokine production capacity at a range of peptide doses

MFI of CD3ζ in CD3 + T lymphocytes of healthy and HNSCC patients was analyzed prior to and following normalization. Prior to normalization, there was no statistically significant difference between the CD3ζ expression in the PBMC of the healthy and HNSCC patients.

2018年11月5日 · We investigated whether a CD33/CD3-bispecific BiTE® antibody construct (AMG 330) with pre-clinical activity against AML-blasts by redirection of T-cells can eradicate CD33 + MDSCs. In fact, T-cells eliminate IDO + CD33 + MDSCs in the presence of AMG 330.

MFIs of CD3 FITC, CD16+CD56 PE, CD45 PerCP-Cy5.5, CD4 PE-Cy7, CD19 APC, and CD8 APC-Cy7 were plotted with time on dates which BD Multi-Check Controls were stained with BD Multitest 6-Color TBNK reagent and acquired on the BD FACSLyric instrument. Over our study period, the mean MFI with CV% of each fluorescence channel was: FITC (2,708, 6.4%), PE