2023年4月14日 · Here, we aimed to remove the multigene family (MGF) 110 gene of unknown function from the Lv17/WB/Rie1 genome to improve the usability of the virus as a live-attenuated vaccine, reducing unwanted side effects. The MGF 110-11L gene was deleted using the CRISPR/Cas9 method, and the safety and efficacy of the virus were tested in pigs after isolation.

2022年12月21日 · We found that ASFV MGF110-7L, a previously uncharacterized member of the multigene family 110, remarkably enhanced the phosphorylation level of eIF2α. In porcine alveolar macrophage 3D4/21 and porcine kidney-15 cells, MGF110-7L triggered eIF2α signaling and the integrated stress response, resulting in the suppression of host translation and ...

2024年7月1日 · In this study, a previously uncharacterized protein, MGF_110-13L, was identified as an immunogenic protein and expressed as a glycosylated homodimer in eukaryotic cells. Two monoclonal antibodies against MGF_110-13L were generated, and two linear epitopes were further identified using monoclonal antibodies.

2023年1月1日 · Among the six early genes, MGF_110–2L and MGF_110–4L were highly related (Fig. 5 B). MGF proteins are widely distributed in ASFVs, but their functions have yet to be well characterized and verified. However, predictions have shown that MGF_110 proteins contained cysteine-rich motifs ideal for oxidizing environments.

2023年7月17日 · 近日，兰州大学动物医学与生物安全学院郑海学教授团队在《The FASEB Journal》正式在线发表了题为“Deletion of MGF-110-9L gene from African swine fever virus weakens autophagic degradation of TBK1 as a mechanism for enhancing type I interferon production”的研究论文。

2021年3月10日 · ASFV MGF-110-9L is a genotype II ASFV that contains approximately 873 nucleotides. The amino acid sequence of ASFV MGF-110-9L is highly conserved among genotype II ASFV. In this study, we demonstrate that deletion of the ASFV gene MGF-110-9L (ASFV-Δ9L and hereafter designated as 9L) from the highly virulent ASFV

2018年10月2日 · The subcellular distribution of multigene family 110 proteins of African swine fever virus is determined by differences in C-terminal KDEL endoplasmic reticulum retention motifs.

Live attenuated viruses that are naturally isolated or genetically manipulated have demonstrated reliable protection against homologous ASFV strain challenge. In the present study, a mutant ASFV strain with the deletion of ASFV MGF-110-9L (ASFV-Δ9L) was generated from a highly virulent ASFV CN/GS/2018 parental strain, a genotype II ASFV.

2021年3月15日 · 本研究从II型非洲猪瘟病毒 CN/GS/2018毒株删除MGF-110-9L基因得到一株缺失毒株（命名ASFV-Δ9L）。 与亲本毒株相比，MGF-110-9L 基因的缺失明显降低了非洲猪瘟病毒在猪巨噬细胞中的体外复制能力。 在21天的观察期内，大多数低剂量肌肉接种ASFV-Δ9L的动物临床正常。 感染ASFV-Δ9L的动物有3/5表现出低的血毒症和高的病毒特异性抗体反应，表明ASFV-Δ9L对猪具有部分致弱作用。 以上的研究将为防控非洲猪瘟提供一定理论依据。 图1 ASFV 9L …

2023年4月14日 · Here, we aimed to remove the multigene family (MGF) 110 gene of unknown function from the Lv17/WB/Rie1 genome to improve the usability of the virus as a live-attenuated vaccine, reducing unwanted side effects. The MGF 110-11L gene was deleted using the CRISPR/Cas9 method, and the safety and efficacy of the virus were tested in pigs after isolation.