Antigen presentation by major histocompatibility complex (MHC) proteins is essential for adaptive immunity. Prior to presentation, peptides need to be generated from proteins that are either produced by the cell’s own translational machinery or that are funneled into the endo-lysosomal vesicular system.

2020年3月11日 · We propose that peptide binding is co-determined by synergy between the binding pockets of the MHC molecule. Major histocompatibility complex (MHC) class I molecules bind peptides in the lumen...

The peptide-binding site of MHC class I molecules contains six pockets of which some are involved in the binding of the peptides.

2017年11月23日 · Here, we isolate human PLC from Burkitt's lymphoma cells using an engineered viral inhibitor as bait and determine the structure of native PLC by electron cryo-microscopy. Two endoplasmic reticulum-resident editing modules composed of tapasin, calreticulin, ERp57, and MHC-I are centred around TAP in a pseudo-symmetric orientation.

2023年8月18日 · Studies of the mechanism by which high-affinity peptides become ligands of MHC I molecules have highlighted that the F pocket at the C-terminal end of the groove is a critical region of ...

2024年4月17日 · To increase attention on important binding pocket features during inception network training, the electrostatic maps for each MHC-I binding pocket was split into three segments: (1) an N-terminal binding pocket region, (2) a TCR contact region, and (3) C-terminal binding pocket region.

2021年6月1日 · Major histocompatibility complex (MHC) class I and class II proteins play an important role in the cell-mediated adaptive immune responses: They present peptides at the cell surface to CD8+ and CD4 + T cells, respectively. MHC proteins are encoded by highly polymorphic genes (called: Human Leukocytes Antigens or HLAs).

H-2Kb, which has a deep C pocket, binds specifically peptides with a tyrosine or a phenylalanine at position 5. In contrast, HLA-A2 has a shallow C pocket, which cannot accommodate large side chains at position 5. Site-directed mutagenesis was used to generate a chimera between the murine H-2Kb and the human HLA-A2 [H-2Kb/HLA-A2 (C')].

3 天之前 · 深入研究发现，MHC分子对抗原肽的识别并非呈现严格的一对一关系，而是一种类型的MHC分子可以识别一群带有特定共同基序的肽段，由此构成MHC分子与抗原肽相互作用的包容性（flexibility）。

2023年6月1日 · In this chapter, we provide an extensive docking protocol for predicting a peptide binding to a given MHC protein. Due to an unusual ligand molecule, a peptide, several types of docking were defined according to flexibility/rigidity of the peptide side chain/s and/or the side chains of the binding pocket/s.