Decoding Human Megakaryocyte Development - ScienceDirect
2021年3月4日 · Here we used single-cell RNA sequencing of human MKs from embryonic yolk sac (YS) and fetal liver (FL) to characterize the transcriptome, cellular heterogeneity, and …
常说的CD3,CD4,CD8,CD19,CD34,CD45 是什么？ - 知乎专栏
CD分子（cluster of differentiation）应用以 单克隆抗体 （McAb）鉴定为主的聚类分析方法，将来自不同实验室的单克隆抗体所识别的同一分化抗原、其编码基因及其分子表达的细胞种类均鉴 …
High-throughput enrichment and isolation of megakaryocyte progenitor ...
2021年4月15日 · (A,B) Flow cytometry plots displaying CD45 and CD41 intensity of bone marrow cells stained with MK antibodies in the (A) − TPO and (B) + TPO conditions. Seven distinct …
CD45 expression discriminates waves of embryonic …
We characterized the precise phenotype of embryo megakaryocytes in the liver at gestational day 11.5, identifying them as CD41 ++ CD45-CD9 ++ CD61 + MPL + CD42c + tetraploid cells that …
CD41+/CD45+ Cells Without Acetylcholinesterase Activity Are Immature ...
2009年1月2日 · GeneChip and semiquantitative polymerase chain reaction analyses revealed that the Lin − /CD41 + /CD45 + /AChE − MKs are assigned at the stage between the progenitor …
Generation of Megakaryocytic Progenitors from Human …
We have established a two step serum-free and stromal cell-free differentiation protocol, initially generating hematopoietic progenitor cells that express CD34, CD41a (hereafter abbreviated to …
CD45 + and CD45 -megakaryocyte subsets are present at
CD45 + and CD45 -megakaryocyte subsets are present at E11.5 in the yolk sac and fetal liver. Flow cytometry studies were performed on preparations from E11.5 fetal liver (FL) cell …
Immunocytochemistry and flow cytometry evaluation of …
1996年3月1日 · Most MK co-expressed CD45 (98.8% positive) but not CD33 (80.7% negative) or CD11b (88.9% negative). Our data indicate that flow cytometry can be used effectively to …
Generation of MKs derived from human induced pluripotent stem …
The suspension cells expressed MK markers CD41 and CD42a and hematopoietic cell markers CD34 and CD45. Ig isotype controls for CD41, CD42a, CD34, and CD45 were used to …
Human marrow megakaryocyte differentiation: multiparameter …
2004年11月1日 · Expression of myeloid CD45 and immunoglobulin G (IgG)-FcγRII receptor (CDw32) increased with megakaryocyte maturation and contrasted with the declining …