2024年11月8日 · Starting from a single MLH1- knockout human colon cell, we modeled the transition of daughter cells and resultant organoids from normal epithelium to carcinoma by sequentially selecting cells...

2024年5月13日 · CT26 Mlh1 KO was sensitive to pan-caspase inhibitor Emricasan, caspase-8 inhibitor Z-IETD-FMK, and RIPK1 inhibitor Nec-1, all of which could inhibit cell death induced by IFN-γ plus TNF-α or ...

2020年2月26日 · Mlh1 knockout (MLH1-KO) cells have increased mutational burden, augmented number of predicted neoantigens, and higher immunogenicity . Because we previously found that MLH1-KO cells grow slower than their parental counterpart in syngeneic mice ( 32 ), we initially injected them into immunocompromised animals until large tumors were established.

2021年1月11日 · In this study, we discovered a hitherto unknown MMR mechanism that modulates genome stability and has implications for cancer therapy. DNA mismatch repair (MMR) maintains genome stability by correcting base mismatches generated during DNA replication. MMR is nick-directed and specifically targets the newly synthesized DNA strand.

While Brca2 L2431P/L2431P MEFs showed basal level of R-loops on Mlh1 +/+ and Mlh1 KO/+ genetic backgrounds, significant increases in R-loops were observed upon Mlh1 loss (Brca2 L2431P/+;Mlh1 KO/KO) . These results clearly suggest that R-loop accumulation could be a major driver for genomic instability and embryonic lethality observed in Brca2 ...

2025年1月17日 · 为了在体外解码其背后的基因突变机理，研究人员选择了类器官模型mlh1 ko 。 此模型通过敲除（KO）MLH1基因，可于 体外 短期培养 中重现d-MMR型CRC突变特征。

2020年7月9日 · Consistently, HORMAD1 KO elevated chromatin binding of MLH1, but not MSH2, in MDAH2774, A549, and HCC38 cells (Figs. 4g, S5C–D). Therefore, HORMAD1 expression compromises DNA mismatch repair by...

本研究探讨了 PIK3CA 状态是否以及如何影响 COX-2 介导的肿瘤炎症和 dMMR CRC 的免疫治疗反应。 使用小鼠结肠癌细胞MC38、CT26和CT26- Mlh1- KO构建PIK3CA敲低和过表达模型来模拟PIK3CA失调的dMMR CRC，并使用异种移植模型评估PIK3CA如何调节COX-2表达、CD8 + T细胞使用免疫活性小鼠进行抗 PD-L1 治疗的浸润、肿瘤生长和治疗反应。 进行蛋白质印迹来描绘人类和小鼠癌细胞中的信号传导途径，并使用人类患者样本进行免疫组织化学分析和生物信息学分 …

2025年1月15日 · In the control cultures (MLH1-KO and MLH1-KO—Empty vector), there was a gradual takeover of MLH1-KO cells, constituting 85% and 90% of the population, respectively (Figure 3A). This observation aligned with a prior study indicating a selective advantage of MLH1-KO pluripotent cells over WT cells .

通过敲除Mlh1基因exon 6，建立Mlh1基因敲除小鼠模型。 *使用本品系发表的文献需注明: Mlh1-KO (2) mice (Cat. NO. NM-KO-232962) were purchased from Shanghai Model Organisms Center, Inc.. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如： …