2025年3月5日 · The identification of novel molecular candidates capable of treating osteoarthritis (OA) has significant clinical implications. Monocyte locomotion inhibitory factor peptide (MLIF) is a pentapeptide derived from Entamoeba histolytica. It has been found possesses selective anti-inflammatory effects both in vitro and in vivo.

Monocyte locomotion inhibitory factor peptide (MLIF) is a pentapeptide derived from Entamoeba histolytica. It has been found possesses selective anti-inflammatory effects both in vitro and in vivo. Nonetheless, like many peptide therapeutics, MLIF has relatively poor proteolytic stability and short half-life in vivo, hindering its effective ...

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产品来源：mLIF 是使用 pGEX 表达系统在大肠埃希菌中表达的带 GST 的融合蛋白，用凝血酶从 GST 部分裂解，并通过多个 FPLC 步骤进行纯化。 白血病抑制因子（LIF）是一种淋巴样因子，可通过抑制自发分化来促进胚胎干细胞的长期维持。 LIF 具有许多其他活性，包括胆碱能神经元分化、干细胞多能性控制、骨骼和脂肪代谢、某些因子依赖性细胞系的有丝分裂，以及在体内对巨核细胞生成的促进。 小鼠LIF是一个22 kDa的蛋白质，含有 203 个氨基酸残基。 1.使用体外半固 …

单核细胞迁移抑制因子(monocyte locomotion inhibitory factor,MLIF)是阿米巴原虫产生的具有抗炎作用的五肽。 体内、外研究发现,MLIF可以发挥抗炎和免疫保护作用,对类风湿关节炎、神经损伤、心肌缺血、脑缺血及痴呆具有良好的保护作用。

单核细胞迁移抑制因子(monocyte locomotion inhibitory factor,MLIF)是阿米巴原虫产生的具有抗炎作用的五肽。 体内、外研究发现,MLIF可以发挥抗炎和免疫保护作用,对类风湿关节炎、神经损伤、心肌缺血、脑缺血及痴呆具有良好的保护作用。

Entamoeba histolytica in culture produces a pentapeptide (MQCNS). This oligopeptide inhibits the in vitro and in vivo locomotion of human monocytes, hence its denomination Monocyte Locomotion Inhibitory Factor (MLIF). The original isolated peptide and its synthetic construct display similar effects, …

Monocyte locomotion inhibitory factor (MLIF) is a heat-stable pentapeptide from Entamoeba histolytica. Our previous study found that MLIF protects against ischemic stroke in rats and mice and exerts a neuroprotection effect in human neuroblastoma SH-SY5Y cells.

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Here we introduced a simple, practical and high level expression of MBP-mouse LIF through Escherichia coli system which was bioactive. The mLIF cDNA was inserted into vector of pMAL-C2X in order to generate N-terminal MBP-mLIF recombinant proteins in the cytoplasm of Escherichia coli. MBP-mLIF as a soluble form was expressed.