Mass Spectrometry for Post-Translational Modifications
Analysis of post-translational modifications (PTMs) by mass spectrometry can be difficult and the level of difficulty is dependent on (i) the mass shift in the peptide molecular weight, (ii) the …
基于质谱的蛋白质翻译后修饰鉴定和定量 - 知乎
蛋白质翻译后修饰 (post-translational modifications, PTMs) 是指在翻译后的蛋白质氨基酸侧链及其C/N末端引入新的基团 (如磷酸基、乙酰基等)。 PTM在细胞分化、蛋白质降解、信号传导和调 …
Mapping protein post-translational modifications with mass
2007年9月27日 · We describe MS strategies used to detect common PTMs, map modified residues and estimate stoichiometries, with particular focus on protein phosphorylation as a …
Detecting diagnostic features in MS/MS spectra of post …
2023年7月12日 · The PTM-Shepherd diagnostic feature mining module aims to perform high throughput identification of spectral features that can be used to identify post-translational …
Mass Spectrometry-Based Detection and Assignment of Protein ...
2014年12月26日 · Here, we provide an update of MS-based PTM analyses, with a focus on current enrichment strategies coupled with revolutionary advances in high performance MS. …
Identification and characterization of post-translational …
2022年8月1日 · Post-translational modifications (PTMs) induce marginal chemical additions to native proteins and lipoproteins after their biosynthesis, generating various isoforms with …
Analysis and Interpretation of Protein Post-Translational …
2019年11月1日 · Protein PTM site stoichiometry is the fraction of proteins that contain a specific PTM at a given site. Thorough understanding of PTM site stoichiometry is valuable for …
LC-MS-MS分析蛋白酶修饰位点 - 知乎 - 知乎专栏
2022年11月1日 · 串联质谱分析是指在一级质谱之后选择特定 m/z 的 母离子 进行二级 MS 分析，可以确定肽段的氨基酸序列以及翻译后修饰的类型和位点。 蛋白酶实质上是一种具有催化 …
蛋白质组学分析革命：什么是 PTMScan？
对于药物开发、突变分析和激酶分析研究，CST 科学家颠覆了传统的抗体开发过程并设计出一种至今仍然推动重要发现的全新 PTM 检测方法：PTMScan。 检测 PTM 的新方法：什么是 …
Decoding Post-Translational Modification Crosstalk With Proteomics ...
2021年1月1日 · PTM crosstalk describes the combinatorial action of multiple PTMs on the same or on different proteins for higher order regulation. Here we review how recent advances in …