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2021年7月22日 · Detailed instructions on reagent preparation and assay protocol for an MTT assay to measure cell proliferation or cell cytoxicity.

The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity; however, it is commonly applied and interpreted erroneously. We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity.

2024年6月14日 · MTT assay is used to measure cell viability, proliferation, and cytotoxicity. Mosmann, T. et al. were the first to describe this non-radioactive, colorimetric test technique using MTT. In living cells, MTT, a yellow tetrazole, is converted to purple formazan.

MTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.

The MTT assay is defined as a method that measures cellular metabolic activity by assessing the reduction of MTT to purple formazan crystals primarily by mitochondrial dehydrogenases, providing insights into cytotoxicity levels in cells.

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity.

Cytotoxicity MTT Assay is a colorimetric assay which measures cell viability, proliferation and cytotoxicity. It involves the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by mitochondrial dehydrogenases in metabolically active cells to form purple formazan crystals.

MTT assay evaluates the effect of a drug on the proliferative index of the cell population. In MTT assay, cells in the exponential phase of growth are exposed to a cytotoxic drug. After addition of the drug, the cells are allowed to proliferate for two to three population-doubling times (PDTs).