A live cell reporter of exosome secretion and uptake reveals ...
2020年4月29日 · Incorporating an additional non-pH-sensitive red fluorescent tag allows visualization of the exosome lifecycle, including multivesicular body (MVB) trafficking, MVB …
MVB-12, a Fourth Subunit of Metazoan ESCRT-I, Functions in …
2007年9月26日 · To test whether MVB-12 requires the presence of other ESCRT-I subunits to associate with membranes in C. elegans, we compared the distribution of GFP:MVB-12 in …
ISGylation controls exosome secretion by promoting lysosomal ...
2016年11月24日 · Despite its co-localization with MVB markers, green fluorescent protein (GFP) was not found in exosomes when fused to ISG15 but rather promoted its accumulation in …
A plant Bro1 domain protein BRAF regulates multivesicular body ...
2018年9月17日 · Here we identify a plant-specific Bro1-domain protein BRAF, which regulates FREE1 recruitment to the MVB/PVC membrane by competitively binding to the ESCRT-I …
Retrofusion of intralumenal MVB membranes parallels viral …
2021年9月13日 · To follow relocalization of membrane components from the intraluminal vesicles (ILVs) back to the limiting membrane (LM), we furnished the canonical multivesicular body …
A Unique Plant ESCRT Component, FREE1, Regulates
2014年11月3日 · Here, we identified a unique plant ESCRT component called FYVE domain protein required for endosomal sorting 1 (FREE1). FREE1 binds to phosphatidylinositol-3 …
Ubiquitin-Dependent Sorting into the Multivesicular Body Pathway ...
2001年7月27日 · The multivesicular body (MVB) pathway is responsible for both the biosynthetic delivery of lysosomal hydrolases and the downregulation of numerous activated cell surface …
Nature子刊：外泌体的活细胞荧光成像用于外泌体分泌和细胞迁移 …
2020年5月6日 · 近日，美国范德堡大学医学院的研究人员在pHluorin中引入了稳定的M153R突变，突变后的pHluorin-CD63表现为更高、更稳定的表达，并能更好地监测外泌体分泌，可能会 …
BPS2025 - Phospholipase D-derived phosphatidic acid regulates ...
2025年2月13日 · This study explores the role of PLD-mediated PA production in the process of multivesicular bodies (MVBs) and plasma membrane (PM) fusion. Using A549 cells co …
ESCRT-I Mediates FLS2 Endosomal Sorting and Plant Immunity
2013年12月26日 · To determine FLS2-GFP location at MVBs, we triggered FLS2 endocytosis and examined sub-compartment localization at enlarged MVBs. We first examined FLS2-GFP …