2020年4月29日 · Incorporating an additional non-pH-sensitive red fluorescent tag allows visualization of the exosome lifecycle, including multivesicular body (MVB) trafficking, MVB fusion, exosome uptake and...

2007年9月26日 · To test whether MVB-12 requires the presence of other ESCRT-I subunits to associate with membranes in C. elegans, we compared the distribution of GFP:MVB-12 in embryos co-depleted of TSG-101 and VPS-4 to that in embryos

2016年11月24日 · Despite its co-localization with MVB markers, green fluorescent protein (GFP) was not found in exosomes when fused to ISG15 but rather promoted its accumulation in detergent-insoluble cell ...

2018年9月17日 · Here we identify a plant-specific Bro1-domain protein BRAF, which regulates FREE1 recruitment to the MVB/PVC membrane by competitively binding to the ESCRT-I component Vps23.

2021年9月13日 · To follow relocalization of membrane components from the intraluminal vesicles (ILVs) back to the limiting membrane (LM), we furnished the canonical multivesicular body (MVB) marker tetraspanin membrane protein CD63 with a GFP tag harboring an N-terminal nuclear localization signal (NLS) and a C-terminal tobacco etch virus (TEV) protease ...

2014年11月3日 · Here, we identified a unique plant ESCRT component called FYVE domain protein required for endosomal sorting 1 (FREE1). FREE1 binds to phosphatidylinositol-3-phosphate (PI3P) and ubiquitin and specifically interacts with Vps23 via PTAP-like tetrapeptide motifs to be incorporated into the ESCRT-I complex.

2001年7月27日 · The multivesicular body (MVB) pathway is responsible for both the biosynthetic delivery of lysosomal hydrolases and the downregulation of numerous activated cell surface receptors which are degraded in the lysosome. We demonstrate that ubiquitination serves as a signal for sorting into the MVB pathway.

2020年5月6日 · 近日，美国范德堡大学医学院的研究人员在pHluorin中引入了稳定的M153R突变，突变后的pHluorin-CD63表现为更高、更稳定的表达，并能更好地监测外泌体分泌，可能会成为了解外泌体自分泌和旁分泌作用的有用工具。 该研究发表于Nature Communications杂志上。 细胞外囊泡（EVs）是从细胞中释放出来的纳米级囊泡，具有强大的自分泌和旁分泌生物学活性。 尽管EV最初被认为是不具有生物学相关性的细胞碎片，但现在EV已被认为构成了一种细胞间通信 …

2025年2月13日 · This study explores the role of PLD-mediated PA production in the process of multivesicular bodies (MVBs) and plasma membrane (PM) fusion. Using A549 cells co-expressing the MVB marker CD63-pHmScarlet and the PA sensor GFP-PASS, we employed real-time total internal reflection fluorescence microscopy to monitor PA dynamics during MVB-PM fusion.

2013年12月26日 · To determine FLS2-GFP location at MVBs, we triggered FLS2 endocytosis and examined sub-compartment localization at enlarged MVBs. We first examined FLS2-GFP endosomes in transiently expressing RFP-ARA7/RabF2b leaf cells .