In eukaryotes, the 5′ cap (cap-0), found on the 5′ end of an mRNA molecule, consists of a guanine nucleotide connected to mRNA via an unusual 5′ to 5′ triphosphate linkage. This guanosine is methylated on the 7 position directly after capping in vivo by a methyltransferase .

The 5′ m7G cap is an evolutionarily conserved modification of eukaryotic mRNA. Decades of research have established that the m7G cap serves as a unique molecular module that recruits cellular proteins and mediates cap-related biological functions ...

真核生物和病毒的mRNA在5'起始端有一个单甲基化修饰的帽子结构，m7G（5'）ppp（5'）G或称为mCAP。 Cap类似物在多种细胞过程中起重要作用，比如蛋白合成的起始，避免核酸外切酶切割RNA，作为招募前体mRNA剪接、聚腺苷酸化和核输出所需蛋白因子的独特地识别信号。

7-Methyl-diguanosine triphosphate (m7Gp3G) 是一种帽类似物，可以整合到 mRNA 中，参与哺乳动物细胞的翻译和 mRNA 降解。 m7Gppm6AmpG 是一种三核苷酸 mRNA 5' 帽类似物，可用于体外 RNA 合成。 m7GpppGmpG 是一种三核苷酸 5′ 端帽类似物。 m7GpppGmpG 对 RNA 的加帽率为 86%。 m7GpppGmpG ammonium 是一种三核苷酸 5′ 端帽类似物。 m7GpppGmpG 对 RNA 的加帽率为 86%。 m7GpppUpG 是一种寡核苷酸，是 M 7 GpppNpG 三核苷酸帽类似物。 …

The eukaryotic translation initiation factor eIF4E directly binds the m 7 G cap and through this interaction plays key roles in many steps of RNA metabolism including nuclear RNA export and translation. eIF4E also stimulates capping of many transcripts through its ability to drive the production of the enzyme RNMT which methylates the G-cap to f...

2017年1月23日 · In this paper, we review approaches employed to develop cap-based molecular tools that could benefit biological studies, with the focus on phosphate-labeled and phosphate-modified cap analogs of m 7 G cap and other structurally related RNA cap structures.

The 5′ methyl G cap is initially recognized by initiation factor eIF4E. Under favorable translation conditions, eIF4G serves as a scaffold bridging the ribosome to the mRNA cap by binding eIF4E, eIF4A, and eIF3.

mRNA of eucaryon and virus has a monomethylated cap structure, m7G(5')ppp(5')G or called mCAP, at the 5’-initiating terminal. The cap analog plays an important role in cellular processes, the initiation of protein synthesis, protecting RNA from exonuclease cleavage, uniquely recognition for recruiting protein factors for pre-mRNA splicing ...

2024年6月25日 · 5′ capping is one of the methods of processing of eukaryotic pre-mRNA. Eukaryotic mRNA has a unique enzymatically appended cap structure consisting of 7- methylguanosine residue joined via a 5′-5′ triphosphate bridge. During transcription, 7- methylguanosine is added to the 5′ end of nascent mRNA.

Eukaryotic mRNA has a 7-methyl-G cap (mCAP) at the 5' end. The cap protects the mRNA from nucleolytic degradation and recruits the ribosomal initiation complex to the start of the mRNA. Translation is dramatically enhanced by the presence of a cap.