2022年2月15日 · MgCl 2 (Magnesium chloride) is an essential ingredient of the PCR master mix. Acting as a cofactor, it enhances the enzymatic activity of DNA polymerase, thereby boosting DNA amplification. Cofactors are non-protein ions or …

2023年9月28日 · Magnesium is a required cofactor for thermostable DNA polymerases and is important for successful amplification. Without adequate free Mg2+, PCR polymerases are not active. In contrast, excess free Mg2+ reduces enzyme fidelity and …

2018年8月6日 · Three important functions of the MgCl 2 in a PCR reaction are, To improve or boost the activity of Taq DNA polymerase. To facilitate effective and accurately complementary primer binding. To increase the melting temperature (compromise the annealing temperature). To increase the amplification efficiency of the reaction.

2023年5月20日 · Magnesium Chloride (MgCl2) is an essential cofactor for optimizing PCR protocols. A concentration range of 1.5 to 4.5 mM of MgCl2 is ideal for creating optimal PCR conditions. Very high or very low magnesium concentrations can both affect PCR negatively.

2020年5月9日 · In PCR, MgCl 2 is an essential cofactor that enhances the activity of Taq DNA polymerase, which in turn increases the amplification rate of DNA. It is important to note, however, that higher concentrations of MgCl 2 can result in decreased specificity.

2014年9月14日 · In PCR, MgCl2 is an essential cofactor that enhances the activity of Taq DNA polymerase, which in turn increases the amplification rate of DNA. It is important to note, however, that higher...

2023年5月20日 · MgCl2 is an essential cofactor that boosts the performance of Taq DNA polymerase in PCR, thereby increasing the rate of DNA amplification. It also enhances the amplification efficiency of the reaction.

For the PCR, adding DMSO (2-10%) when amplifying the randomized region of the tRNA is highly recommended to prevent secondary structure formation (Varadaraj & Skinner, 1994).

一般来说，对以DNA或cDNA为模板的PCR反应，应选择2－5 mM浓度的MgCl2，对以mRNA为模板的RT－PCR而言，则应选择的浓度为4－8 mM。

2018年4月26日 · Magnesium chloride is the preferred method of adding magnesium to a PCR experiment. Thermostable polymerase requires the presence of magnesium to act as a cofactor during the reaction process.