The first enzyme of the PET degradation pathway, PETase, cleaves this plastic into the intermediates MHET (Mono-(2-hydroxyethyl)terephthalic acid) and minor amounts BHET (Bis-(2-hydroxyethyl)terephthalic acid). MHETase hydrolyses the ester bond of MHET forming terephthalic acid and ethylene glycol. Enzymatic PET degradation by PETase and MHETase

2019年4月12日 · Two α/β-hydrolase fold enzymes (α/β-hydrolases), PETase and MHETase, work together to degrade PET in two steps via MHET, yielding TPA and ethylene glycol—the building blocks required for a ...

PETases are an esterase class of enzymes that catalyze the breakdown (via hydrolysis) of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl terephthalate (MHET). The idealized chemical reaction is: (ethylene terephthalate) n + H 2 O → (ethylene terephthalate) n-1 + MHET,

2023年11月9日 · A study on the computational design of enzymes for plastic (MHET and PET) degradation yielded an efficient dual enzyme degradation system for PET recycling with a substantially increased...

2021年8月6日 · In 2016, one of the two enzymes involved in the polyethylene terephthalate (PET) degradation pathway of Ideonella sakaiensis 201-F6, MHETase, was found to exhibit a strong ability to degrade the PET monomer mono-(2-hydroxyethyl)terephthalate (MHET) at room temperature, converting it back into the precursors used in PET production. MHETase ...

Ideonella sakaiensiscells bind to the PET surface and degrade it into mono(2-hydroxyethyl) terephthalic acid (MHET), a heterodimer of terephthalic acid (TPA) and ethylene glycol, using a secreted PET hydrolase, or PETase. The first PETase was discovered in I. sakaiensis, and it works by hydrolyzing the ester bonds found in PET with high ...

PET的酶水解产生对苯二甲酸 (TPA)、乙二醇 (EG)、对苯二甲酸单 (2-羟乙基)酯 (MHET)和对苯二甲酸双 (2-羟乙基)酯 (BHET)的混合物作为主要产品。 这项工作开发了一种新的方法来量化生物催化剂的水解活性，使用 BHET 作为模型底物。 该协议可用于筛选用于 PET 解聚反应的酶，以及其他应用。 非常好的拟合（R 2 = 0.993) 实验数据和数学模型之间证实了 Michaelis-Menten 方程分析 BHET 浓度 (8-200 mmol L -1 ) 对特异腐质霉 角质酶 (HiC)催化的初始水解速率影响的可行 …

2018年1月26日 · Based on structural and site-directed mutagenesis experiments, the detailed process of PET degradation into MHET, terephthalic acid, and ethylene glycol is suggested.

2022年9月15日 · The recently reported PET hydrolase (DuraPETase) and MHET hydrolase (MHETase) can synergistically catalyze the complete PET degradation. Hence, this work was designed to develop a bienzymatic cascade catalysis …

2022年11月10日 · The enzymatic hydrolysis of PET results in a mixture of terephthalic acid (TPA), ethylene glycol (EG), mono-(2-hydroxyethyl) terephthalate (MHET) and bis-(2-hydroxyethyl) terephthalate (BHET) as main products.