2025年1月17日 · Du and colleagues model early embryonic development using mouse totipotent blastomere-like cells (mTBLCs). This system captures key events such as cell fate determination, compaction, and polarization. Single mTBLCs efficiently form blastoids and develop into post-implantation egg-cylinder-like structures in vitro and in vivo.

The mouse embryo—tiny and inaccessible in its mother's womb—presents a hard challenge to developmental biologists. It has, however, two immediate attractions. First, the mouse is a mammal, and mammals are the animals that we, as humans, care about most.

5 天之前 · Mice and cells. Embryonic limb cells were derived from embryos at embryonic day 13.5. Adult brain and lung cells were derived from ~4–6-week-old WT or transgenic mice. Postnatal brain, lung ...

1 天前 · Introduction Pluripotent stem cells (PSC) are known to recurrently acquire genetic aberrations including chromosomal aneuploidy during long-term culture of which the consequences remain largely unknown. A recent study reported that xenografting of aneuploid mouse embryonic stem cells (mESC) gives rise to teratomas containing undifferentiated elements with metastatic capacity. This contrasts ...

2025年3月20日 · Lodewijk et al. develop a CRISPRa-programmed embryo model using mouse pluripotent cells. By introducing controllable CRISPR activation tools into pluripotent cells, they enabled the efficient generation of a reproducible model. These tools were also used to disrupt developmental pathways, offering a method to study mechanisms of developmental disorders.

2024年4月25日 · In this study, we introduce an optimized blastocyst complementation technique that allows for the efficient screening of candidate genes and streamlines the generation of functional rat embryonic stem cell (rESC)-derived forebrain tissues in mice.

6 天之前 · One well-known rare cell type in mouse visual cortex is the chandelier cell, which synapses onto the AIS of excitatory neurons 20,34,35,36,37,38. We used a single proofread chandelier cell to ...

Mouse embryonic stem cells (mESCs) were first isolated and propagated in culture in 1981. mESCs are typically isolated from blastocysts from the inner cell mass of 3.5-day-old embryos and have the potential to generate every cell type found in the body.

Here we highlight the importance of mice in stem cell biology and in bringing the world one step closer to seeing these cells brought to fruition in modern medicine. Mouse embryonic stem cells (ESCs) were first isolated by in vitro culture of cells isolated from the inner cell mass (ICM) of early embryos or blastocysts 1,2 (Fig. 1).

2008年11月20日 · In this review, we will discuss a range of methods for characterizing HSCs, progenitors, and mature hematopoietic cells, which can then be applied to the analysis of mutant mice or nonsteady state conditions.