mKate2 is a basic (constitutively fluorescent) red fluorescent protein published in 2009, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity. mKate2 was derived from mKate with the following mutations: M1_S2insV/V45A/M146T/S158A/K231R.

第一种方式是，将荧光示踪基因通过2A（自剪切多肽）或IRES（内部核糖体进入位点序列）敲入到小鼠内源基因的3'端，对内源基因表达进行示踪（如图5所示）；第二种方式是，将荧光基因敲入到小鼠内源基因的5'端，通过终止序列polyA与内源基因连接，可以实现内源基因的替代表达，通过观察荧光示踪基因的表达了解内源性蛋白的表达情况，例如：是否表达、表达量、表达组织分布等等。 图5. 用于蛋白表达谱研究的小鼠模型构建示意图. 例如，为寻找激活褐色脂肪细胞的小分 …

mKate2 is the superior fluorescent monomeric tag for imaging in living tissues. It has emission maximum at 635 nm optimal for deep imaging of animal tissues, and is more bright, photostable and pH-stable than other cloned far-red monomeric fluorescent proteins.

Remarkably, mKate2 is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including mKate, mRaspberry and mPlum (Figures 2d and 2e and Table 1). In contrast with mKate, mKate2 demonstrates almost no kindling (photoactivation) phenomena, consistent with the structural considerations discussed above.

Monomeric far-red fluorescent protein. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. The 1-base overhangs produced by BmrI may be hard to ligate. Sticky ends from different BmrI sites may not be compatible. Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.

与商业 mKate2 相比，双突变体 mKate2-K67R/R197H（又名 FusionRed2 和 Diogenes）的荧光亮度增加了约 1.6 倍，代表了下一代超亮红色荧光探针，为荧光成像提供了新的可能性。

目的 制备远红荧光蛋白报告基因mKate2慢病毒,用于标记人肝癌细胞株HepG2,并进行体外的细胞荧光成像,为肿瘤的活体荧光成像示踪提供基础.方法 以pmKate2-N质粒为模板,将mKate2基因克隆到慢病毒表达载体pLenti6.3/V5-DEST;pLenti6.3-mKate2表达质粒和包装质粒共转染293T细胞 ...

mKate2 is the superior fluorescent monomeric tag for imaging in living tissues. It has emission maximum at 635 nm optimal for deep imaging of animal tissues, and is more bright, photostable and pH-stable than other cloned far-red monomeric fluorescent proteins.

mKate2 is the next generation of far-red fluorescent protein TagFP635 (mKate) [Shcherbo et al., 2007; Shcherbo et al., 2009]. Possessing fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5.

Here, we report the generation of a transgenic mouse line, called mito::mKate2, in which the mitochondria are ubiquitously labeled with the mKate2 fluorescent protein.