2017年4月6日 · Here, we show that, during necroptosis, MLKL-dependent calcium (Ca 2+) influx and phosphatidylserine (PS) exposure on the outer leaflet of the plasma membrane preceded loss of PM integrity. Activation of MLKL results in the generation of broken, PM “bubbles” with exposed PS that are released from the surface of the otherwise intact cell.

mlkl是tnf（肿瘤坏死因子）诱导的坏死性凋亡过程中的关键靶标，它作为ripk1和ripk3下游的效应蛋白发挥作用。 RIPK3在Ser227位点被磷酸化，此磷酸化为MLKL激活所必需。

21066-1-AP targets MLKL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Proteintech's Rabbit Recombinant Phospho-MLKL (Ser358) antibody is validated in WB, ELISA and shows reactivity with human samples.

ZBP1/RIP3/MLKL Activator 1 induces DNA damage, enhances intracellular levels of reactive oxygen species (ROS), and triggers apoptosis via the caspase pathway. Furthermore, when apoptosis is inhibited, ZBP1/RIP3/MLKL Activator 1 promotes necroptotic cell death through the ZBP1-RIP3-MLKL pathway.

2017年7月3日 · ripk3对mlkl的磷酸化进一步增强ev产生，这个过程不同于细胞死亡途径之，促使磷酸化的mlkl分子进入到ev中，从而促进细胞生存。 这一结果发表在最新的Immunity杂志上。

2024年7月19日 · RIPK3激活后，会进一步磷酸化并激活混合谱系激酶样结构域（MLKL）。 MLKL 是坏死性凋亡的执行者。 被激活的MLKL会转位至细胞膜，插入并形成孔道，导致细胞膜的破裂和细胞内容物的泄露。 对该信号通路进行总结： 死亡受体激活 （如TNF受体）→ RIPK1激活并与RIPK3结合 形成坏死小体→ RIPK3磷酸化MLKL → MLKL转位至细胞膜 并形成孔道→ 细胞膜破裂 和细胞内容物泄露→炎症反应. 坏死性凋亡信号通路的调控机制和临床 相关性 仍在研究中，其 …

2020年6月29日 · 作者又用NSA封住MLKL和膜互作的位点，发现NSA抑制了MLKL的寡聚、膜转位、pMLKL聚集成hotspots，最终抑制了膜损伤，且NSA加入的越早抑制作用越强，暗示MLKL和膜的互作对于MLKL诱导程序性坏死很重要

Mixed lineage kinases (MLKs) are mitogen activated protein kinase kinase kinases (MAPKKKs) with features of both serine-threonine and tyrosine kinases that regulate the c-Jun N-terminal kinase (JNK) mitogen activated protein kinase (MAPK) signaling cascade, and also regulate p38 and extracellular signal-regulated kinase (ERK).

30145-1-AP targets Mlkl in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.