Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. [5] This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity.

2024年12月25日 · MLKL (Mixed Lineage Kinase Domain Like Pseudokinase) is a Protein Coding gene. Diseases associated with MLKL include Maturity-Onset Diabetes Of The Young and Caspase 8 Deficiency. Among its related pathways are RIPK1-mediated regulated necrosis and Ion channel transport.

We identified UBA52 (ubiquitin A-52 residue ribosomal protein fusion product 1) as the binding partner of MLKL under physiological conditions. Loss of Mlkl induced a decrease in ubiquitin levels by preventing UBA52 cleavage.

2023年8月3日 · Mixed Lineage Kinase domain-Like pseudokinase (MLKL) is implicated in a broad range of diseases due to its role as the ultimate effector of necroptosis and has therefore emerged as an attractive drug target. Here, we describe the development of PROteolysis TArgeting Chimeras (PROTACs) as a novel approach to knock down MLKL through chemical means.

2025年2月8日 · Mixed Lineage Kinase Domain-Like Pseudokinase (MLKL) Gene Expression in Human Atherosclerosis with and without Type 2 Diabetes Mellitus. Location, location, location: A compartmentalized view of TNF-induced necroptotic signaling. PI3K mediates tumor necrosis factor induced-necroptosis through initiating RIP1-RIP3-MLKL signaling pathway activation.

2022年3月1日 · Pseudokinase mixed lineage kinase domain-like protein (MLKL) serves as a terminal-known obligate effector in the process of necroptosis. To date, the majority of research on MLKL has focused on its role in necroptosis, and the prevailing view has been that the sole function of MLKL is to mediate necroptosis.

2017年7月18日 · Activation of the pseudokinase mixed lineage kinase domain-like (MLKL) upon its phosphorylation by the protein kinase RIPK3 triggers necroptosis, a form of programmed cell death in which rupture of cellular membranes yields release of intracellular components.

Mixed lineage kinase domain-like (MLKL) protein is the main player of this cell death pathway as it mediates the final step of plasma membrane permeabilization. Despite the significant progress in our knowledge of the necroptotic pathway and MLKL biology, the precise mechanism of how MLKL functions remain unclear.

MLKL是坏死的刽子手，一个RIPK3依赖的过程。 坏死可能作为一种肿瘤抑制机制，并作为凋亡抵抗的备用. 2021年5月20日讯/ 生物谷 BIOON/---比利时根特大学研究者在Cell Death & Differentiation杂志上发表了题为"MLKL in cancer： more than a necroptosis regulator"的综述。 MLKL是坏死的刽子手，一个RIPK3依赖的过程。 坏死可能作为一种肿瘤抑制机制，并作为凋亡抵抗的备用细胞死亡方式。 坏死会引发炎症，刺激肿瘤生长。 一些癌症通过RIPK3的表观沉默 …

2020年6月29日 · MLKL的行为追踪的差不多了，着手看它和细胞死亡的关系，先看看细胞死亡过程：TSI刺激细胞后，细胞膜出现损伤（Annexin V着色），损伤积累到一定程度后膜破损，TOPRO进入细胞. 在细胞死亡过程中可以观察到Annexin V出现聚集，对MLKL染色后发现Annexin V聚集在pMLKL形成的hotspots处，暗示pMLKL hotspots处是膜损伤处（和前面的MLKL行为串到一起，分子链条逐渐呈现） 很有意思，作者还观察到了死亡传递：一个细胞发生程序 …