Monobody: 蛋白质靶向治疗的明日之星 - 知乎 - 知乎专栏
Monobody是一种通过筛选技术获得的小分子工程蛋白质，首先由芝加哥大学（现于纽约大学朗格尼医学中心）的Shohei Koide博士提出并发明。 Monobody是基于 三型纤连蛋白结构域 …
Monobody - Wikipedia
Monobodies are synthetic binding proteins constructed using a fibronectin type III domain (FN3) as a molecular scaffold. Specifically, this class of binding proteins are built upon a diversified …
Teaching an old scaffold new tricks: Monobodies constructed …
We diversified positions on a β-sheet and surface loops that together form a concave surface. The new library produced binding proteins (termed “monobodies”) to multiple target proteins, …
Monobodies as tool biologics for accelerating target validation …
Genetically encoded monobodies are effective “tool biologics” for validating intracellular targets. They promote crystallization and help reveal the atomic structures of the monobody-target …
Monobodies as enabling tools for structural and mechanistic biology
Monobodies lack disulfide bonds and thus they are particularly suited as genetically encoded reagents to be used intracellularly. This article reviews recent monobody-enabled studies that …
Development of light-responsive protein binding in the monobody …
2020年8月13日 · Monobodies are synthetic non-immunoglobulin customizable protein binders invaluable to basic and applied research, and of considerable potential as future therapeutics …
Koide Laboratory – Synthetic Protein Science
Monobodies are synthetic binding proteins that are constructed using a fibronectin type III domain (FN3) as a molecular scaffold. Monobodies are simple and robust alternative to antibodies in …
Design and Synthesis of Monobody Variants with Low …
2023年10月9日 · In this study, we designed and synthesized a novel monobody variant with two cysteine substitutions that facilitate the synthetic process via sequential native chemical …
Monobodies and other synthetic binding proteins for expanding …
2017年3月1日 · The difference between the natural PPI and a synthetic PPI is even more dramatic in the case of H-RAS. Monobody NS1 binds to a homodimerization interface of H …
Nat Commun | 非共价选择性靶向和降解RAS突变体 - 知乎
研究人员通过monobody合成技术合成了一种 单克隆抗体 12VC1，能够识别激活状态的KRAS（G12V）和KRAS（G12C），对 野生型 KRAS的选择性高达400倍。晶体结构表 …
伊利诺伊大学研发靶向Ras蛋白monobody，有望治疗30%肿瘤
2016年11月14日 · Monobody本质上是类似抗体的合成结合蛋白，根据设计以及结构上的不同，能够和酶、受体等许多蛋白质结合。Monobody发挥作用不依赖于环境，可以被当做是蛋白质版 …
Monobody adapter for functional antibody display on ... - Nature
2022年10月11日 · Here, the authors improve the targeting efficiency of antibodies on nanoparticles using a monobody adapter to correctly orientate the antibody to preserve …
Monobodies as tool biologics for accelerating target validation and ...
Genetically encoded monobodies are effective “tool biologics” for validating intracellular targets. They promote crystallization and help reveal the atomic structures of the monobody-target …
Targeted Protein Degradation through Cytosolic Delivery of Monobody …
2019年4月26日 · Monobodies are small engineered binding proteins that, upon expression in cells, can inhibit signaling of cytosolic oncoproteins with outstanding selectivity. Efficacy may …
Monobodies and other synthetic binding proteins for expanding …
Among synthetic binding protein platforms, the most established systems include Affibodies, Anticalins, Monobodies and DARPins [Fig. 1 (B)]. Affibodies are based on the Z domain of …
Monobodies as enabling tools for structural and mechanistic biology
2020年2月1日 · Monobodies lack disulfide bonds and thus they are particularly suited as genetically encoded reagents to be used intracellularly. This article reviews recent monobody …
Monobodies as possible next-generation protein therapeutics – a ...
Up to now, targeted cancer therapeutics come in two main flavours: therapeutic monoclonal antibodies (mAbs) target the extracellular side of cell surface receptor proteins on tumour cells …
Development of light-responsive protein binding in the monobody …
2020年8月13日 · Monobodies are synthetic non-immunoglobulin customizable protein binders invaluable to basic and applied research, and of considerable potential as future therapeutics …
Characterization of monobody scaffold interactions with ligand …
2015年2月4日 · Monobodies have been engineered to bind a wide variety of target proteins with high affinity and specificity. Using alanine-scanning mutagenesis simulations, we identified two …