2009年6月1日 · 由于克隆了两种人类酪氨酸蛋白磺基转移酶（tpst-1 和 tpst-2）、新的分析和合成方法的发展以及对含有磺基酪氨酸残基的蛋白质和肽的详细研究，我们对蛋白质酪氨酸硫酸化的理解取得了最新进展。

Tyrosylprotein sulfotransferase (TPST) is a 54- to 50-kDa integral membrane glycoprotein of the trans-Golgi network found in essentially all tissues investigated, catalyzing the tyrosine O-sulfation of soluble and membrane proteins passing through ...

Tyrosylprotein sulfotransferase is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins.

Arabodopsis TPST is a 500-residue type I transmembrane proteins with a 24-residue N-terminal signal peptide (SP), a 446-residue luminal catalytic domain, followed by a single TM and a short cytoplasmic domain. Potential N-glycosylation sites are depicted as lancets.

酪氨酸硫酸盐化是由两种称为酪氨酰蛋白质磺基转移酶（TPST）1和2的高尔基驻留酶催化的，它们将辅酶PAPS（3'-磷酸腺苷5'-磷酸硫酸盐）中的硫酸盐转移至蛋白质底物中的上下文相关酪氨酸。 缺乏定量的酪氨酸硫酸化测定法已经阻碍了用于鉴定酪氨酸硫酸化的小分子抑制剂的化学生物学方法的发展。 在本文中，我们描述了针对TPST1和TPST2的基于非放射性迁移率的酶促测定方法的发展，通过该方法可以快速定量合成荧光肽的酪氨酸硫酸化。 我们利用配体结合和抑制剂 …

Tyrosine sulfation is catalysed by two Golgi-resident enzymes termed tyrosylprotein sulfotransferases (TPSTs) 1 and 2, which transfer sulfate from the cofactor PAPS (3′-phosphoadenosine 5′-phosphosulfate) to a context-dependent tyrosine in a protein substrate.

In Arabidopsis, tyrosine sulfation is essential for the activities of peptide hormones, such as phytosulfokine (PSK) and root meristem growth factor (RGF). Here, we identified a TPST-encoding gene, MtTPST, from model legume Medicago truncatula. MtTPST expression was detected in all organs, with the highest level in root nodules.

2018年8月14日 · Tyrosine sulfation is catalysed by two Golgi-resident enzymes termed tyrosylprotein sulfotransferases (TPSTs) 1 and 2, which transfer sulfate from the cofactor PAPS (3'-phosphoadenosine 5'-phosphosulfate) to a context-dependent tyrosine in a protein substrate.

为了研究植物中酪氨酸硫酸化的机制，我们从拟南芥 MM2d 细胞的微粒体部分纯化了 TPST 活性，并鉴定了一种 62-kDa 的蛋白质，该蛋白质与 PSY1 前体肽的硫酸化基序特异性相互作用。

2014年8月15日 · Motivation: Tyrosine sulfation is a type of post-translational modification (PTM) catalyzed by tyrosylprotein sulfotransferases (TPST). The modification plays a crucial role in mediating protein-protein interactions in many biologically important processes.