2013年9月1日 · NAC (N-acetyl-L-cysteine) is commonly used to identify and test ROS (reactive oxygen species) inducers, and to inhibit ROS. In the present study, we identified inhibition of proteasome inhibitors as a novel activity of NAC. Both NAC and catalase, another known scavenger of ROS, similarly inhibited R …

Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine)是ROS抑制剂，拮抗多种蛋白酶体抑制剂的活性。 它还是肿瘤坏死因子 TNF 的抑制剂，主要用作祛痰剂，可通过维持或恢复肝脏中谷胱甘肽的浓度来处理扑热息痛(对乙酰氨基酚过量)。

Acetylcysteine (N-Acetylcysteine) is a mucolytic agent which reduces the thickness of the mucus. Acetylcysteine is a ROS inhibitor. Acetylcysteine is a cysteine precursor, prevents hemin-induced ferroptosis by neutralizing toxic lipids generated by arachidonate-dependent activity …

NAC是常用的细胞ROS清除剂，但是按照文献上给的浓度10mM和5mM作用细胞24h，细胞都死掉了，不知是什么原因？ 使用这个药物的时候是否有必要做一下MTT，摸一下最佳使用浓度？

2021年11月23日 · N-acetylcysteine (NAC), often used as an antioxidant-scavenging reactive oxygen species (ROS) in vitro, was recently shown to increase the cytotoxicity of other compounds through ROS-dependent and ROS-independent mechanisms. In this study, NAC itself was found to induce extensive ROS production in h …

N-acetylcysteine (NAC) is an aminothiol and synthetic precursor of intracellular cysteine and GSH and is thus considered an important antioxidant 4. NAC has been widely used as a research tool in the field of apoptosis research for investigating the role of ROS in induction of apoptosis.

肝细胞受损伤时（药物，酒精，毒物等各种因素）产生大量活性氧(ROS)可使肝内促氧化物增多，抗氧化物减少，发生氧化应激，引起肝内三磷酸腺苷衰竭和GSH水平下降。 NAC能通过影响肝组织GSH，SOD，血红素氧化酶(hemeoxygenase-1, HO-1)活力来清除ROS保护肝细胞。

N‑acetylcysteine (NAC) is a thiol‑containing antioxidant that modulates the intracellular redox state. NAC can scavenge reactive oxygen species (ROS) and maintain reduced glutathione (GSH) levels, in order to protect cardiomyocytes from oxidative stress. The present study aimed to determine whether …

查了好久关于NAC的使用经验，好多板块都有不同的问题和方法，今天硬着头皮做了实验，结果是可以有效抑制ROS。 把过程发出来给大家参考和提供建议。 材料：NAC抗氧化剂，购自碧云天，2g装，用去离子水24ml稀释，终浓度510mM（为方便计算，具体实验中当500mM用了），充分溶解后用滤器过滤作为原液，分装-20℃冻存备用。 测了酸碱度，强酸，为了不影响其抗氧化作用就没调，而且说明书也没说要调PH嘛。 ROS检测用碧云天的活性氧检测试剂盒，染料 …

N-acetyl-L-cysteine (NAC)：NAC是一种ROS抑制剂，可通过提供还原型的硫氢化合物，减少ROS的生成。 NAC在一些研究中被用于减轻细菌对ROS的响应，如降低氧化应激对细菌生长的影响。