2020年10月1日 · NHE1 flux was found to be activated by low [NO], but inhibited at high [NO]. These responses involved cGMP-dependent signalling, rather than S-nitros(yl)ation. Stronger cGMP signals, that can inhibit phosphodiesterase enzymes, allowed [cAMP] to rise, as demonstrated by a FRET-based sensor.

2021年2月21日 · This mini review focuses on the ubiquitous isoform 1 (NHE1) of this protein family also known as the “cardiac isoform” and its regulation by the cyclic guanosine monophosphate/protein kinase G (cGMP/PKG) signaling route, triggered by inhibition of the phosphodiesterase 5A (PDE5A) by Sildenafil.

The sodium/hydrogen exchanger-1 (NHE1) is the most important pH-regulatory transporter in cardiomyocytes. 2 A reduction of pH i activates NHE1 to extrude H + and leads to H +-activated Na + influx. The rise in intracellular Na + in turn reduces the driving force for operation of the Na + /Ca 2+ exchanger for Ca 2+ extrusion and results in ...

2022年8月15日 · Isoform one (NHE1) is present on the plasma membrane and regulates intracellular pH by removal of one intracellular proton in exchange for one extracellular sodium thus functioning as an electroneutral process. Human NHE1 has a 500 amino acid membrane domain plus a C-terminal 315 amino acid, regulatory cytosolic tail.

NHE1是恩格列净在心肌细胞和血管平滑肌细胞唯一表达的靶蛋白，恩格列净抑制心肌细胞的Na + /H + 交换，Na + 降低，抑制钙超载，保护线粒体结构、功能的完整

2003年6月1日 · NHE1 is the most predominant isoform expressed in heart where it contributes to cardiomyocyte pH homeostasis. Although the NHE activation is essential for the restoration of physiological pH, hyperactivation of NHE1 during ischemia–reperfusion episodes disrupts the intracellular ion balance, leading to cardiac dysfunction and damage.

NHE1 is a Na + /H + exchanger responsible for keeping intracellular pH (pH i) balance and cell growth. Our study aimed to investigate roles of NHE1 in high glucose (HG)-induced apoptosis in renal tubular epithelial cells. Renal epithelial tubular cell line HK-2 was cultured in medium containing 5 mM or 30 mM glucose.

2021年6月10日 · 研究人员利用冷冻电镜单颗粒技术，解析了人源NHE1-CHP1复合物在pH7.5条件下、pH6.5条件下的内向构象和pH7.5条件下小分子药物Cariporide结合外向构象的高分辨率结构。 人源NHE1呈现同源二聚体形式，单体包含13个跨膜螺旋，可被划分为二聚化结构域（TM1-3，7-10）和核心结构域（TM4-6，11-13）。 二聚化结构域和核心结构域的相对运动，介导了膜两侧钠离子和质子的交换。 CHP1与NHE1的C端两亲性螺旋HC1结合，并在NHE1的内向和外向构象 …

et al. (63) reported two different routes for cGMP-triggered NHE1 regulation according to NO concentration. Using an aminoacidic sequence that reproduced the C-terminal of the

2021年2月1日 · Inferring from the actions of membrane-permeant analogues, cGMP was determined to activate NHE1, whereas cAMP was inhibitory, which explains the biphasic regulation by NO.