Differential activation of Tat variants in mitogen-stimulated …
2003年5月25日 · Mutations (Fig. 1) were introduced into the wild-type Tat sequence present in the NL4-3 infectious clone (Chen et al., 1994). This allele is identical to HXB2 Tat apart from the …
NL4-3 mCherry Luciferase - Addgene
Plasmid NL4-3 mCherry Luciferase from Dr. Warner Greene's lab contains the insert mCherry, Luciferase, IRES and is published in PLoS One. 2012;7(1):e30176. doi: …
Enhanced NF-κB activation via HIV-1 Tat-TRAF6 cross-talk
2024年1月19日 · The knockdown of TRAF6 in Jurkat cells also reduced p24 production after infection with the NL4-3 (Tat-SF) virus (fig. S4C). In the infection experiments, the virally …
PRMT2 promotes HIV-1 latency by preventing nucleolar exit and
2023年11月10日 · Here, through a targeted complementary DNA (cDNA) expression screening, we identify PRMT2 as a key suppressor of Tat activation, thus contributing to proviral latency …
A novel, sensitive dual-indicator cell line for detection and ...
2019年12月18日 · Here we describe the construction and validation of a novel dual-indicator cell line, Sup-GGR, which offers two different readouts to quantify viral replication. A construct …
pBS NL4-3 envFS eGFP 5'CMV Δtat - Addgene
Plasmid pBS NL4-3 envFS eGFP 5'CMV Δtat from Dr. Jeremy Luban's lab contains the insert NL4-3 and is published in Nat Commun. 2018 Dec 13;9 (1):5305. doi: 10.1038/s41467-018 …
Splicing Regulatory Elements within tat Exon 2 of Human ...
In the NL4-3 strain of human immunodeficiency virus type 1 (HIV-1), regulatory elements responsible for the relative efficiencies of alternative splicing at the tat, rev, and the env/nef 3′ …
CRISPR/Cas9 system targeting regulatory genes of HIV-1 ... - Nature
2018年5月17日 · Our design selection of highly conserved tat and rev sequences was based on the HXB2 reference strain, and throughout this study, the constructs were shown to be …
Latently-Infected CD4+ T Cells are Enriched for HIV-1 Tat Variants …
2009年4月4日 · TZM-bl cells were transfected with 0.1µg of either empty vector, NL4-3 Tat, a clone containing the consensus Tat sequence (the most common sequence for that …
A mutant Tat protein inhibits infection of human cells by strains …
2017年3月14日 · To evaluate Nullbasic inhibition of Tat-mediated transactivation of different HIV-1 strains, we infected TZM-bl-NB-mCh and TZM-bl-mCh cells with HIV-1 NL4–3 (subtype B), …