Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals additional insights into the protein interaction network of the spliceosome.

PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe . Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536

2025年1月20日 · Here, we report two cryo-electron microscopy structures of post-B act spliceosome intermediates from Schizosaccharomyces pombe primed for disassembly. We identify the DEAH-box helicase–G-patch...

2017年9月21日 · In this paper, we report the cryo-EM structure of the S. cerevisiae ILS complex at an average resolution of 3.5 Å, which allows identification of the disassembly proteins Prp43, Ntr1/Spp382, and Ntr2 and the splicing factor Cwc23. Analysis of the structural features reveals important insights into the disassembly mechanism of the ILS complex.

Although S. pombe contains no clear Ntr2 homolog, it is of note that Ntr1, Prp43, and Brr2 were all significantly substoichiometric in our purified complexes (Table 1). During spliceosome acitivation, Brr2 functions to unwind the U4/U6 helices to enable release of U4 snRNA and formation of the catalytic core in which U6 is extensively base ...

2019年6月20日 · Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides...

pombe. Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals addi-

2022年3月18日 · Here, we present a protocol for tandem affinity purification (TAP) of protein complexes from the fission yeast Schizosaccharomyces pombe. The protocol employs cells expressing C-terminally TAP-tagged proteins and is suitable for the analysis of purified proteins by mass spectrometry.

Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals additional insights into the protein interaction network of the spliceosome.

The recruitment of Ntr2 and Ntr1 into the spliceosome serves two purposes: to ensure the correct spliceosomal complex is chosen for disassembly and to activate the helicase Prp43. The presence of Ntr2 and Ntr1 also facilitates the recruitment of Prp43. Prp43 may act by pulling either the intron lariat or the 3′ sequences of U6 snRNA.