One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation. Is NdeI a Time-Saver™ Qualified enzyme? Is NdeI activity sensitive to dam, dcm or mammalian CpG methylation? Does NdeI have any isoschizomers?

2025年3月1日 · 高甘油浓度、Mn2+存在、高离子强度条件下，识别序列会发生变化。 用该酶切出的突出末端的连接效率低，所以必须使用平滑末端的连接条件。 该酶稀释后不稳定，请使用原液。 如果在反应液中添加Triton X-100 (终浓度0.01%)，相对活性上升到150%。 识别位点 制品内容 酶浓度10U/μl 附带缓冲液反应缓冲液H反应停止液10×LoadingBuffer 制品说明 起源EscherichiacolicarryingtheplasmidencodingNdeIgene 反应温度37℃ 活性测定用底物λDNA Star …

Nuclear distribution protein nudE homolog 1 is a protein that in humans is encoded by the NDE1 gene. [5] [6] [7]

2024年12月25日 · NDE1 (NudE Neurodevelopment Protein 1) is a Protein Coding gene. Diseases associated with NDE1 include Lissencephaly 4 With Microcephaly and Microhydranencephaly. Among its related pathways are Loss of proteins required for interphase microtubule organization from the centrosome and EML4 and NUDC in mitotic spindle formation.

2025年2月9日 · Comprehensive analysis indicated that NDE1 is a potential biomarker for pan-cancer and promotes bladder cancer progression. Novel lissencephaly-associated NDEL1 variant reveals distinct roles of NDE1 and NDEL1 in nucleokinesis and human cortical malformations.

2023年11月9日 · Here, we investigate how human Nde1 and Lis1 regulate the assembly and subsequent motility of mammalian dynein using in vitro reconstitution and single molecule imaging. We find that Nde1...

In this review, we have explored the roles of Nde1 in progenitor cell behavior and mitosis, the genotype–phenotype relationships of NDE1 associated developmental disorders, and most importantly, we identified relationships between the pattern of NDE1 terminal exon usage and the development of highly gyrencephalic cortices.

该基因编码核分布 E (NudE) 蛋白家族的成员。 编码的蛋白质位于中心体并与其他中心体成分相互作用，作为调节动力蛋白功能的多蛋白复合物的一部分。 这种蛋白质在微管组织、有丝分裂和神经元迁移中起着重要作用。 该基因的突变会导致无脑回畸形 4，这是一种以无脑回畸形、严重的脑萎缩、小头畸形和严重的认知障碍为特征的疾病。 可变剪接导致多个转录本变体。 [RefSeq 提供，2012 年 3 月] This gene encodes a member of the nuclear distribution E (NudE) family of …

2024年11月2日 · This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration.

大肠杆菌菌株，其携带反硝化奈瑟菌（Neisseria denitrificans）（NRCC 31009）的 NdeI 基因。 一个单位是指在 50 µl 的总反应体系中，37℃ 条件下，1 小时内酶切 1 µg λ DNA 所需的酶量。 对 CpG、dcm 或 dam 甲基化均不敏感。 Why Choose Recombinant Enzymes? Is NdeI a Time-Saver™ Qualified enzyme? Is NdeI activity sensitive to dam, dcm or mammalian CpG methylation? Does NdeI have any isoschizomers?