2020年5月15日 · Newcastle disease virus (NDV) has been explored to a great extent to understand the biology of negative-sense RNA viruses. Nucleoprotein (N) is the most abundant protein in the virus particles, and its primary function is to encapsidate the virus genome for its transcription, replication, and packaging.

2025年2月20日 · Herein, we successfully developed a recombinant Newcastle disease virus with porcine α1,3GT gene (NDV-GT) triggering hyperacute rejection. We demonstrated its feasibility in preclinical studies. The intravenous NDV-GT showed superior ability to eradicate tumor cells in our innovative CRISPR-mediated primary hepatocellular carcinoma monkeys.

2023年3月10日 · Newcastle disease virus (NDV) belongs to Paramyxoviridae, which contains lethal human and animal pathogens. NDV RNA genome is replicated and transcribed by a multifunctional 250 kDa...

2023年9月1日 · To examine the ultrastructural properties of the NDV IBs, the standard transmission electron microscopy (TEM) of infected cells was performed. DF-1 cells were infected with F48E9 at an MOI of 0.1 and fixed for TEM analysis at 24 h post-infection.

2020年5月15日 · Here, we report the structural investigations of the N protein of NDV (NDV-N) in solution. The N gene of NDV was cloned and expressed in E. coli as a soluble protein of ~53 kDa in size. The FE-TEM imaging of the purified NDV-N displayed a nearly spherical shape with a diameter of 28 nm and the DLS analysis of the purified NDV-N displayed a ...

2013年7月11日 · We demonstrated that NDV infection induced steady-state autophagy in chicken-derived DF-1 cells and in primary chicken embryo fibroblast (CEF) cells, evident through increased double- or single-membrane vesicles, the accumulation of green fluorescent protein (GFP)-LC3 dots, and the conversion of LC3-I to LC3-II.

... electron microscopy (TEM) analysis of rNDV-infected Vero cells. Overall, areas in which budding virions accumulated were observed more commonly in the cells infected with rSG10-F23A (Fig. 4A).

Transmission electron microscopy (TEM) observation on purified rNDV-IBV-T/B and LaSota strains. (A) The 20th passage (P20) of rNDV-IBV-T/B, which appears as circles or ovals about 100 to 150 nm...

2023年1月25日 · In this study, the paramyxovirus Newcastle disease virus (NDV) was employed to study the FPIV L-domain. Two recombinant NDV strains, each carrying a single amino acid mutation at the Phe (F23) or Pro (P24) site of 23 FPIV/I 26 L-domain, were rescued. Growth defects were observed in only the recombinant SG10-F23A (rSG10-F23A) strain.

2021年10月31日 · To further identify cellular energy sensor signaling pathways and autophagic occurrences during NDV infection, we first utilized transmission electron microscopy (TEM) to observe it. Notably, NDV infection resulted in an increased number of mitochondria that were trapped by double or single membrane vesicles ( Figure 6B ).