Neurospheres: a potential in vitro model for the study of
2021年3月25日 · Two main rich sources of these cells are the subventricular zone (SVZ) and the subgranular zone of the hippocampal dentate gyrus (SGZ) where adult neural stem cells (aNSCs) have the ability to proliferate and differentiate into mature cell lines.
The neurosphere assay: an effective in vitro technique to study …
Recently, our group demonstrated the potential of an in vitro technique established more than two decades ago, the neurosphere assay (NSA) to obtain high yields of NSCs from the main neurogenic niches and to study the inherent properties of the NSCs.
Neurosphere - an overview | ScienceDirect Topics
A neurosphere is a 3-dimensional, free-floating colony that forms in the presence of mitogens and consists of hundreds of cells of which a small percentage (1–5%) are stem cells and the remainder are progenitors (Morshead et al., 1994, 2002).
Neurosphere formation of human neural stem cells (hNSCs) is a widely used in vitro culture system and valuable model to study neurogenesis and neural development. This system allows for three-dimensional (3D) expansion of hNSCs within a more phys-iologically relevant microenvironment.
In this review, we describe the principles of neurosphere assay and analyze the diferent aspects of research with neural stem cells, highlighting the main implications of an in vitro model that can help explain the outcomes of some neurodegenerative diseases.
Neural stem cells and neurospheres—re-evaluating the ... - Nature
2005年4月21日 · Although a variety of techniques were initially used, the neurosphere assay (NSA) 3, 6 rapidly emerged as the assay of choice and has since become a valuable tool for isolating, and understanding...
Production of Neurospheres from CNS Tissue - PMC - National …
Neurospheres can be cultivated from a variety of normal, genetically altered, or pathological tissue specimens, even with protracted postmortem intervals, for studies of mechanisms underlying neurogenesis, cell fate decisions, and cell differentiation.
Defining the actual sensitivity and specificity of the neurosphere ...
2006年9月21日 · We asked whether neurospheres are clonal structures whose diameter, number and composition accurately reflect the proliferation, self-renewal and multipotency of a single founding NSC.
The Neurosphere Simulator: An educational online tool for …
2021年1月1日 · Neurospheres are spherical structures that are formed, under serum-free culture conditions, by proliferation of single neural stem or progenitor cells when stimulated with epidermal growth factor, fibroblast growth factor 2, or both (Reynolds and Weiss, 1992).
Neurospheres: insights into neural stem cell biology - PubMed
2004年12月15日 · In this review, the importance of maintaining cell-cell contacts in the study of NSC is highlighted, and data obtained from some crucial single-cell studies is compared to results obtained from neurospheres, where aggregates of NSC are grown in suspension.
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