Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA. It is called "non-homologous" because the break ends are directly ligated without the need for a homologous template, in contrast to homology directed repair (HDR), which requires a homologous sequence to guide repair.

2020年4月3日 · Imprecise NHEJ mediated repair can produce insertion and/or deletion mutations of variable length at the site of the DSB. HDR-mediated repair can introduce precise point mutations or insertions from a single-stranded or double stranded DNA donor template.

2023年10月8日 · 非同源末端连接（NHEJ）检测系统包含一个启动子、嘌呤抗性、GFP； 启动子通过一个puro基因与GFP编码盒分离，puro基因两侧是两个具有相同方向的I-SceI位点，当加入I-Sce I核酸内切酶时，puro基因被切除，NHEJ修复两个I-SceI诱导的DSB，I-SceI识别位点之间的末 …

2017年5月17日 · Mammalian non-homologous DNA end joining (NHEJ) is the primary pathway for the repair of DNA double-strand breaks (DSBs) throughout the cell cycle,...

The two major pathways for repair of DNA double-strand breaks (DSBs) are homologous recombination (HR) and nonhomologous end joining (NHEJ). HR leads to accurate repair, while NHEJ is intrinsically mutagenic. To understand human somatic mutation it ...

DNA双链断裂修复主要通过 非同源末端连接 （Non-homologous End Joining, NHEJ）和 同源重组 （Homologous Recombination, HR）来完成，其中NHEJ容易出错并引入突变而HR具有高度的精确性，因此HR对基因组稳定性的维持尤为重要。近期研究通过检测HR核心蛋白RAD51在损伤位点 …

2018年11月26日 · Imprecise NHEJ mediated repair can produce insertion and/or deletion mutations of variable length at the site of the DSB. HDR-mediated repair can introduce precise point mutations or insertions from a single-stranded or double stranded DNA donor template.

Nonhomologous DNA end-joining (NHEJ) is the predominant double-strand break (DSB) repair pathway throughout the cell cycle and accounts for nearly all DSB repair outside of the S and G2 phases. NHEJ relies on Ku to thread onto DNA termini and ...

2008年9月15日 · DNA double-strand breaks (DSBs) are dangerous lesions that can lead to potentially oncogenic genomic rearrangements or cell death. The two major pathways for repair of DSBs are nonhomologous end joining (NHEJ) and homologous recombination (HR). NHEJ is an intrinsically error-prone pathway while HR r …

2023年6月19日 · 非同源末端连接 （Non-homolouous end joining，NHEJ） 和同源重组 （Homologous recombination，HR） 是DSB修复的两条主要通路。 一般地，HR被认为是DNA高保真的修复方式，但在特定情况下，如对于富含重复序列的异染色质来说，HR修复则容易造成非等位基因间同源重组 （non-allelic homologous recombination，NAHR），从而导致染色体的重排与畸变。 因此，细胞如何调控和选择这两种途径对DNA修复的精确性以及基因组稳定性的维持 …