2020年9月30日 · Notably, the OGD/R-induced decrease in viability (CCK-8 OD) (Fig. 2b) and cell death (LDH medium release, Fig. 2c) were significantly attenuated by miR-422a inhibition.

2023年2月15日 · Tectoridin improved cell viability and reduced the release of LDH damaged by OGD/R. A. Chemistry-based structure exhibited by tectoridin. B. PC12 cell was cultured by using tectoridin at different concentrations (0.781–200 μM) for 24 h and MTT method was used to analyze cell viability. C. The protective effect of tectoridin (3.125, 6.25, 12. ...

1.2.5. 细胞培养液中LDH水平的检测. 采用分光光度法检测细胞培养液中LDH的水平以反映细胞中LDH的漏出率。收集各组细胞的培养液，按照LDH检测试剂盒说明书，使用酶标仪测定450 nm波长处的吸光度值，以control组或TAN+OGD组LDH为100%，计算其他各组的LDH漏出率。

Reperfusion following shorter periods (4 and 6 h) of OGD recovered apoptotic PC12 cells, whereas after 12 h, OGD increased LDH release. In primary neurons, 6 h OGD led to significant increase in cytotoxicity, reduction in MTT activity and dendritic MAP2 staining. Reperfusion following 6 h OGD increased the cytotoxicity.

2015年8月21日 · LDH generated after stretch, OGD, a combination of both and successive reoxygenation was measured. LDH was expressed as a percent of the total releasable LDH (LDH in media plus cells). Data are mean values ± SEM, n = 3. *, ¤, $ p < 0.05 compared to no stretch, stretched, and OGD, respectively. OGD, oxygen glucose deprivation; RO, re-oxygenation.

LDH inhibition reduced cell survival and the extracellular levels of lactate in either normoxia or OGD-exposed cells, possibly due to the LDH committals in NAD + recycling, which may have undermined the redox state and the lactate/pyruvate ratio. Indeed, lactate-mediated neuroprotection seems conditional to microglial and neuronal lactate ...

Astrocytes were randomly divided into control group, OGD/R group, and OGD/R+carnosine group (1, 3, 9 mmol/L). The morphological changes of cells were observed by inverted microscope. The cell survival rate was detected by MTT assay. Lactate dehydrogenase (LDH) detection kit was used to detect the leakage of LDH.

2023年5月5日 · 乳酸脱氢酶（ldh）释放实验是另一种常用的细胞毒性筛选方法。这种方法是通过检测培养液中乳酸脱氢酶的活性来评估细胞膜损伤程度，进而反映细胞死亡率。您可以尝试使用ldh释放实验作为补充方法，与cck-8实验结果相对照，以更全面地评估药物的治疗效果。

Results: Melatonin enhanced the viability and reduced the death and LDH release of OGD/R exposed SH SY5Y cells. Melatonin repressed the HMGB1, TNF-α, and iNOS mRNA expression, NO production, and nuclear factor κB (NF-κB) activation in OGD/R challenged SH SY5Y cells. Melatonin reduced the ROS, MDA, 4-HNE, and 8-OHdG contents but further ...

2016年3月1日 · OGD performed for 18 h was followed by re-oxygenation for 10 and 20 h to determine the optimum period of re-oxygenation. Cells cultured under normal conditions during the experimental period served as the control. The lactate dehydrogenase (LDH) (Beyotime Biotechnology, Beijing, China) assay was used to measure cytotoxicity.