
Evaluating the glucose tolerance test in mice
To determine whether consciousness would affect glucose tolerance, we conducted OGTT in mice that were either conscious or anesthetized, using a fixed dose of 50 mg glucose/mouse. …
Evaluating the glucose tolerance test in mice - PubMed
Glucose tolerance was most different and therefore significant (P = 0.001) in high-fat-fed mice after 6 h of fasting (1,973 +/- 96 vs. 1,248 +/- 83 mmol.l(-1).120 min(-1)). The difference in …
Guidelines and Considerations for Metabolic Tolerance Tests in Mice
Nagy C, Einwallner E. Study of in vivo glucose metabolism in high-fat diet-fed mice using Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT). J Vis Exp. …
Oral Glucose Tolerance Test (OGTT): Measuring Glucose …
2023年4月30日 · Pick up the mouse by the back of the neck and stroke the tail a few times to ensure sufficient blood flow to the tail region. Using a new razor blade, cut off a small piece of …
Oral Glucose Tolerance Test | Taconic Biosciences
An oral glucose tolerance test (OGTT), in which the mice are challenged with a bolus of glucose and blood glucose and insulin levels are measured across a two hour time course is …
The Glucose Tolerance Test in Mice - PubMed
Here, we describe in detail how to perform a GTT using four different routes of glucose administration: intraperitoneal, oral, voluntary oral, and intravenous. Keywords: Beta-cell; …
The Oral Glucose Tolerance Test (oGTT) measures the clearance of an orally administered glucose bolus from the body. It is used to detect disturbances in glucose metabolism that can …
The glucose tolerance test in mice: Sex, drugs and protocol
Adult male and female C57Bl/6J mice, implanted with HD‐XG glucose telemetry devices, were fasted for 16 hours or 6 hours following acute habituation stress due to whole cage change, …
Oral Glucose Tolerance Test in Mouse.protocols.iohttps://dx.doi.org/10.17504/protocols.io.ujjeukn. This protocol details the …
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice …
Here, we present a protocol for the generation and metabolic characterization of high-fat diet (HFD)-fed mice as a frequently used model of diet-induced insulin resistance. We illustrate in …