2021年9月21日 · We show that, in virtual absence of proliferation, RPE cells stretch and flatten, thereby matching the retinal curvature and promoting OV folding. Localized interference with the RPE cytoskeleton disrupts tissue stretching and OV folding.

2012年10月16日 · We previously described the essential role of the transmembrane protein Opo, encoded by the gene ojoplano/Ofcc1, during optic cup morphogenesis. Opo regulates the asymmetric localization of focal adhesion components to the basal surface of the retina epithelium and consequently, ojoplano loss of function impairs basal constriction in the ...

2009年12月1日 · Ojoplano (Opo) is a morphogenetic gene playing an important role during embryogenesis in medaka. This report focuses on the identification and characterization of the mouse Opo gene. We examined Opo expression by whole-mount in situ hybridization and in situ hybridization on sagittal sections during mouse embryogenesis.

Mutant analysis in medaka (Oryzias latipes) has identified the vertebrate-specific transmembrane protein Opo, encoded by the gene ojoplano (opo), as an essential component of the molecular...

2021年8月1日 · During this process, different tissue domains execute distinct morphogenetic programs: the distal layer, or the prospective retina, compacts and folds along its basal surface; the medial layer, or the prospective retinal pigmented epithelium (RPE), flattens and covers the retina; and at the boundary, medial layer cells move around the rim to ...

2010年1月1日 · The late optic vesicle becomes patterned into distinct ocular tissues: the neural retina, retinal pigment epithelium (RPE), and optic stalk. Multiple congenital eye disorders, including anophthalmia or microphthalmia, aniridia, coloboma, and retinal dysplasia, stem from disruptions in embryonic eye development.

2020年9月23日 · We show that, in virtual absence of proliferation, RPE cells stretch into a squamous configuration, thereby matching the curvature of the underlying retina. Forced proliferation and localized interference with the RPE cytoskeleton disrupt its …

We suggest that the yapnl13 mutation results in an RPE-specific downregulation of cell contractility which affects the correct folding of the optic cup resulting in failure of choroid fissure...

Ojoplano encodes a transmembrane protein that localizes basally in the retinal neuroepithelium, and antagonizes the Numb/Numbl pathway-mediated endocytosis of Integrin . As a consequence, ojoplano mutants display increased integrin-β1 internalization, which is proposed to affect transmission of cortical tension and cell shape changes across ...

We show that Opo regulates the polarized localization of focal adhesion components to the basal cell surface. Furthermore, tissue-specific interference with integrin-adhesive function impairs optic cup folding, resembling the ocular phenotype observed in opo mutants.