Orcein is a reddish-brown dye, orchil is a purple-blue dye. Orcein is also used as a stain in microscopy to visualize chromosomes, [8] elastic fibers, [9] Hepatitis B surface antigens, [10] and copper-associated proteins. [11] Orcein is not approved as a food dye (banned in Europe since January 1977), with E number E121 before 1977 and E182 after.

Orcein was originally a natural dye obtained from lichens, but is now synthetically produced. It can be used to stain for elastic fibres and Hepatitis B Surface antigens. Demonstration of Hepatitis B surface antigens acts by oxidation of sulphur containing proteins by Potassium permanganate, which form reactive sulphonate residues.

2022年7月7日 · Orcein. The orcein stain is used to identify the inclusion bodies of viruses. These are viral particles within human cells and are visible using light microscopy, unlike viruses themselves. The Orcein stain is commonly used to diagnose hepatitis B, which causes inclusion body formation in hepatocytes.

Orcein was originally obtained from lichens, but is now made from orcinol by hydrogen peroxide oxidation with ammonia present. It is a standard dye to use for the demonstration of elastic fibres, when it is applied in an acid, alcoholic solution.

Current uses of orcein to demonstrate elastic fibers and, following permanganate oxidation (Shikata's modification), hepatitis B surface antigen, copper associated protein, and sulfated mucins, are reviewed. Variations in staining performance with batch of dye and age of dye solution is also discuss …

Orcein is a naturally occurring vegetable dye, which has now been synthesized. Variations between batches of dye may produce erratic results with insufficient depth of stain on occasions. The main advantage of this stain is the simplicity of preparation. The choice of fixative appears unimportant. Amandine Wahart, ... Laurent Duca.

Orcein Dye Staining of Elastic Fibers. Orcein is a complex mixture of related compounds originally derived from lichens but now made synthetically. The synthetic product is often considered to be superior in staining abilities to the natural product and is invariably the material provided from laboratory supply companies.

PRINCIPLE: Orcein stains the inclusion bodies, gives a “ground glass” appearance. CONTROL: A known hepatitis positive liver (carrier). A known copper control. FIXATIVE: Any well fixed tissue. TECHNIQUE:Cut paraffin sections 4-5m. EQUIPMENT:Coplin jars, rinse all glassware in D.I. water. REAGENTS: Potassium Permanganate.

The dry dye Orcein for microscopy Certistain ® is used for the preparation of a staining solution for staining of elastic fibers. It is also used for sex chromatin staining, nuclear staining and demonstration of hepatic inclusions, especially of hepatitis B antigen.

Acetic orcein staining of polytene chromosomes was introduced in 1941 (1) shortly after the initial studies on aceto-carmine-stained chromosomes by Bridges (2) and has remained a standard method of preparation. Orcein dye can be purchased in both its natural form as...