I discovered the green fluorescent protein GFP from the jellyfish Aequorea aequorea in 1961 as a byproduct of the Ca-sensitive photoprotein aequorin (Shimomura et al., 1962; Johnson et al., 1962), and identified its chro-mophore in 1979 (Shimomura, 1979). GFP was a beautiful protein but it remained useless for the next 30 years after the discovery.

The Nobel Prize in Chemistry 2008 was awarded jointly to Osamu Shimomura, Martin Chalfie and Roger Y. Tsien "for the discovery and development of the green fluorescent protein, GFP" To cite this section

He was awarded the Nobel Prize in Chemistry in 2008 for the discovery and development of green fluorescent protein (GFP) with two American scientists: Martin Chalfie of Columbia University and Roger Tsien of the University of California-San Diego.

2018年10月19日 · 绿色萤光蛋白(Green fluorescent protein，简称GFP)，是一个由约238个氨基酸组成的蛋白质，从蓝光到紫外线都能使其激发，发出绿色萤光。 GFP的荧光非常稳定，在激发光照射下，其抗 光漂白 能力比荧光素强很多。因此GFP及其变种今日被广泛地用作分子标记。

Osamu Shimomura painstakingly isolated GFP from hundreds of thousands of jellyfish, characterized the chromophore and elucidated the mechanism of Aequorean bioluminescence. Martin Chalfie expressed the protein in E. coli and C. elegans, and Roger Tsien developed a palette of fluorescent proteins that could be used in a myriad of applications.

2008年10月8日 · In the early 1960s, Osamu Shimomura collected raw material from thousands of these jellyfish and extracted a small amount of a blue luminescent protein, which was subsequently named aequorin....

2018年11月13日 · He discovered green fluorescent protein (GFP), with which — decades later — biomedical researchers began to monitor the workings of proteins in living tissue, and to confirm the insertion of...

Discovery of green fluorescent protein (GFP) (Nobel Lecture) ... Author Osamu Shimomura 1 Affiliation 1 Marine Biological Laboratory, Woods Hole, MA 02543, USA. [email protected]; PMID: 19579247 DOI: 10.1002/anie.200902240 No …

2018年10月19日 · Because GFP can be linked to other proteins thanks to genetic engineering, it has become an important tool for studying biological processes in cells. The first steps in achieving this were taken by Osamu Shimamura, who isolated GFP from the jellyfish Aequorea victoria in the 1960s and found that the protein glowed green when illuminated with ...

Osamu Shimomura was the first person to isolate GFP and to find out which part of GFP was responsible for its fluorescence. His meticulous research laid the solid foundations on which the GFP revolution was built.