Gene expression patterns revealed that OsGDH1, OsGDH2, and OsGDH4 are expressed ubiquitously in various tissues, whereas OsGDH3 expression is glumes and stamens specific. The expression of the OsGDH family members responded differentially to nitrogen and phosphorus-deprivation, indicating their roles under such stress conditions.

Dec 1, 2020 · In the meantime, as demonstrated by qPCR experimental results, OsGLK, OsGDH, OsNR, and OsGS has been increased significantly, which demonstrated by enhancing gene transcription, welan gum regulates the expression of key enzymes involved in nitrogen metabolism, thus improving the ability of rice seedlings to absorb and utilize nitrogen. 5.

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May 9, 2009 · Identification of rice GDH gene family. To obtain GDH gene family in rice, the protein family ID PF02812 was queried in TIGR database. This search identified four GDH-like protein genes, which are named OsGDH1 to OsGDH4 (Table 1).Three of the OsGDH genes have one or two differently spliced transcripts, with sequences collected in the databases (Table 1 and Supplemental Table 2); the ...

Jun 1, 2009 · The expression of the OsGDH family members responded differentially to nitrogen and phosphorus-deprivation, indicating their roles under such stress conditions. Implications of the expression ...

3．在无氮（n-）或无磷（p-）条件下osgdh基因家族各成员的表达谱变化互不相同，表明其在这些胁迫条件下的功能也可能各不相同。 4．层次聚类分析（hca）表明只有osgdh2和osgdh4这两个基因才与gs和gogat这些已知氮代谢过程中基因聚集到一起。 通过hca我们还是不能 ...

Oct 1, 2005 · Abstract. In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH 4 + ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots.

May 16, 2019 · Expression analysis of OsGDH gene in roots and shoots of seedlings exposed to CNO − from qRT-PCR test. Values are mean of four independent biological replicates. Vertical lines represent standard deviation. Asterisk symbol refers to the significant difference between treatment and control (p < 0.05). Control refers to the non-treated rice ...

and His-TF-OsGDH proteins and enzyme assays in vitro The cDNA fragments encoding the MgGDH and OsGDH proteins were amplified by PCR from the optimized pGWC-MgGDH vector and the rice (Oryza sativa L. cv. ‘kitaake’) cDNA, respectively, and then cloned into the pCold-TF vector (TaKaRa, Shiga, Japan) with a Trigger

May 4, 2015 · In AtGDH transgenic rice leaves, endogenous OsGDH expression was slightly lower than empty control (Fig 4F); however, the expression levels of endogenous genes related to AsA biosynthesis in the other transgenic plants were quite similar to those of the empty control (Fig (Fig4D 4D and and4E). 4E). The results of this analysis suggested that ...