2023年11月24日 · Here, we induced dedifferentiation of intestinal epithelial cells using OSKM (Oct4, Sox2, Klf4, and c-Myc) in vivo. The OSKM-induced forced dedifferentiation showed similar molecular features of intestinal regeneration, including a transition from homeostatic cell types to injury-responsive–like cell types.

2014年2月13日 · Increased expression of Lgr5 was similarly observed in the transplanted secondary tumors (Figure S3D). Therefore, we established iPSC lines from OSKM-inducible MEFs containing Lgr5-EGFP reporter allele in which Lgr5 expression can be visualized by enhanced green fluorescent protein (EGFP) (Barker et al., 2007).

2024年1月23日 · SSEA1-negative cells were found to express LGR5 (dubbed LGR5-AdOSKM), and these cells responded to 3 cross-signaling pathways—IL6/Jak/Stat3, LGR5/R-spondin, and Wnt/β-catenin in both 2D and 3D long-term culture conditions.

2024年4月15日 · Following engraftment in syngeneic mice, LGR5-positive cells that expressed the cancer markers CD51, CD166, and CD73 were capable of forming invasive and metastatic tumors reminiscent of intrahepatic cholangiocarcinoma (ICC): they were positive for CK19 and CK7, featured associations of cord-like structures, and contained cuboidal and atypical ...

The LGR5 +-derived tumors exhibited a highly vascularized stroma with substantial fibrosis. In addition, we identified pro-angiogenic factors and signaling pathways involved in neo-angiogenesis and vascular development, which represent potential new targets for anti-angiogenic strategies to overcome tumor resistance to current ICC treatments.

2023年11月24日 · Here, we induced dediferentiation of intestinal epithelial cells using OSKM (Oct4, Sox2, Klf4, and c-Myc) in vivo. The OSKM-induced forced dediferentiation showed similar molecular features of intestinal regeneration, including a transition from homeostatic cell types to injury-responsive–like cell types.

2017年10月1日 · Short-term induction of OSKM in vivo ameliorates aging-associated phenotypes and elongated lifespan. However, premature termination of in vivo reprogramming also leads to cancer development. The expression of OSKM in vivo simultaneously induces cellular senescence, indicating the complexity of in vivo reprogramming.

2017年5月5日 · Additionally, the Wnt target gene leucine-rich-repeat containing G-protein-coupled receptor (Lgr5), which marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles (Barker et al., 2008), can be induced to form small Lgr5 + liver stem-like cells capable of generating ...

2024年4月1日 · We observed that, under long-term 2D and 3D culture conditions, hepatocytes could be converted into LGR5-positive cells with self-renewal capacity that was dependent on 3 cross-signaling pathways: IL6/Jak/Stat3, LGR5/R-spondin, and Wnt/β-catenin.

2022年12月15日，Carina Biotech宣布已提交其治疗实体瘤的 LGR5 CAR-T疗法的IND申请，主要是针对晚期结直肠癌患者的1/2a期临床试验。 LGR5又称G蛋白偶联受体49 (GPR49)或G蛋白偶联受体67 (GPR67)，是GPCR A类受体蛋…