Using otsA, the trehalose biosynthesis gene, and a mutant strain, we demonstrated that otsA confers Burkholderia with competitiveness when establishing a symbiotic association with bean bugs, especially playing a role in initial infection stage. In vitro assays revealed that otsA provides the resistance against osmotic stresses. Hemipteran ...

↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner) ; ↑ Kaasen, I et al. (1994) Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Gene 145 9-15 PubMed; ↑ 3.0 3.1 Baba, T et al. (2006) Construction of ...

The Ots gene cluster of Escherichia coli encodes the synthetic apparatus for the formation of alpha,alpha-1,1-trehalose, a non-reducing glucose disaccharide. The otsA gene encodes a trehalose-6-phosphate synthase, a glycosyltransferase which catalyses the synthesis of alpha,alpha-1,1-trehalose-6-pho …

2021年4月1日 · The otsB gene was found to have highest similarity between bacterial species compared to the otsA gene. Based on phylogenetic analysis, S. qinglanensis and S. lincolnensis were found to be clustered together for otsA and otsB genes.

The enzyme OtsA (trehalose-6-phosphate synthase) is ubiquitous in both prokaryotic and eukaryotic organisms, where it plays a critical role in stress resistance and glucose metabolism. Here, we cloned the otsA gene from Arthrobacter sp. Cjts, and expressed and then purified the recombinant proteins. …

2024年12月17日 · Gene ID: 946405, updated on 17-Dec-2024. Summary. Under conditions of high osmolarity, Escherichia coli synthesizes high concentrations of internal trehalose. [More information is available at EcoCyc: EG11751]. Other designations. trehalose-6-phosphate synthase. GeneRIFs: Gene References Into Functions

2021年1月21日 · On phylogenetic analysis, the otsA and otsB genes of S. qinglanensis was found to be highly conserved among the bacterial species. The otsB gene was found to have highest similarity between bacterial species compared to the otsA gene.

2020年1月1日 · Expression analysis of genes otsA, P5CR, glgX, major chaperone; groELS, dnaKJ, and nodulation protein-coding nodC genes were carried to reveal their roles in enhancing the PEG-induced drought tolerance.

2004年6月16日 · The Ots gene cluster of Escherichia coli encodes the synthetic apparatus for the formation of α,α-1,1-trehalose, a non-reducing glucose disaccharide. The otsA gene encodes a trehalose-6-phosphate synthase, a glycosyltransferase which catalyses the synthesis of α,α-­1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor.

Using otsA, the trehalose biosynthesis gene, and a mutant strain, we demonstrated that otsA confers Burkholderia with competitiveness when establishing a symbiotic association with bean bugs, especially playing a role in initial infection stage.