Invitrogen Anti-Phospho-IRE1 alpha (Ser724) Polyclonal, Catalog # PA5-105424. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL).

Phospho-IRE1 (Ser724) Antibody detects endogenous levels of IRE1 only when phosphorylated at phospho S724. 引用格式: Affinity Biosciences Cat# AF7150, RRID:AB_2843590. Unconjugated. The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator.

2024年5月8日 · 肌醇需要酶1（Inositol-requiring enzyme 1，IRE1）是位于内质网上的跨膜蛋白，通过激活其胞内段蛋白激酶和核糖核酸内切酶的双重活性，起始并控制着未折叠蛋白响应中最为保守的一条信号通路。

2020年8月8日 · p-IRE1α接下来会催化一个重要的过程：使Xbp1 mRNA发生剪切，形成一个功能活跃的转录因子Xbp1s。 Xbp1s进入细胞核后会启动一系列基因转录(如脂质合成、ERAD等)，从多个层次缓解内质网应激。

Upon activation of UPR, IRE1α splices X-box binding protein (XBP1) mRNA by an unconventional mechanism using its endoribonuclease activity. This converts XBP1 into a potent transcriptional activator that induces many UPR responsive genes.

IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.

Western blot analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.