p16 is expressed by astrocytes, while p21 is associated with both neurones and astrocytes. Expression of p16 was detected in the nucleus and cytoplasm of glial cells but not in neurones. Cytoplasmic staining was mainly localized to star-shaped small cells, which resembled reactive astrocytes and their processes (Figure 1).

To address the relationship between senescent cell accumulation and brain aging, we designed studies to eliminate highly p16 Ink4a ‐expressing cells through pharmacogenetic (INK‐ATTAC mouse model) and senescent cells through pharmacologic (senolytic drugs: Dasatinib and Quercetin (D + Q)) approaches. We hypothesized that removal of ...

2024年11月11日 · Our data demonstrate that CSS induces a p16Ink4a -dependent increase in key senescence markers in select organs in male mice, while CRS increases p21 Cip1 exclusively in the brain of male but not...

We screened human primary and recurrent malignant glioma, juvenile pilocytic astrocytoma, medulloblastoma, and meningioma tissue specimens for alterations in p16 gene structure. Single strand conformation polymorphism (SSCP) analysis was used to screen for point mutations, and a quantitative polymer …

2022年6月1日 · In the aging brain, senescence is evident in the glia and the post-mitotic neurons. Glial and neuronal senescence is mediated by p53/p21, p16/RB, and NF-κB pathways. The senescence of brain cells is a major risk factor for neurodegenerative diseases. Emerging senolytics have shown promising outcomes in ameliorating neurodegeneration.

衰老的脑髓细胞在体外促进外周免疫细胞趋化性。活化的驻留和浸润免疫细胞在老年大脑中增加，并通过雌性 p16-InkAttac 小鼠的 p16 阳性衰老细胞清除部分恢复到年轻水平，这与认知功能的保存有关。

2024年3月16日 · We found that p16 ink4a expression was increased in microglia near amyloid plaques in brain tissue from patients with AD and 5XFAD mice, a model of AD. In BV2 microglia, small interfering RNA (siRNA)-mediated p16ink4a downregulation transformed microglia with enhanced amyloid phagocytic capacity through regulated the cell cycle and increased ...

2006年9月9日 · We have addressed this question by examining progenitor frequency, proliferation and neurogenesis in the forebrain lateral ventricle subventricular zone (SVZ) of ageing wild-type and p16INK4a -deficient mice. The SVZ contains a mixed population of stem cells and other progenitors that engage in neurogenesis throughout adult life 6, 13, 22.

2021年10月1日 · Single-cell RNAseq of brain cells indicated p16 High cells were primarily microglia, and their accumulation was confirmed in brains of aged humans. Interestingly, we identified two distinct subpopulations of p16 High microglia in the mouse brain, with one being age-associated and one present in young animals.

2022年9月27日 · Activated resident and infiltrating immune cells increase in the aged brain and are partially restored to youthful levels through p16-positive senescent cell clearance in female p16-InkAttac...