p62 耗竭使 Tsc2-null 细胞对氧化应激和丁硫氨酸亚砜亚胺对 GSH 生物合成的直接抑制敏感。我们的研究结果表明 p62 如何帮助维持细胞内 GSH 池，以限制 mTORC1 升高的肿瘤细胞中的线粒体功能障碍，突出 p62 和氧化还原稳态作为这些肿瘤治疗靶向的节点脆弱性。

2025年2月1日 · Mechanically, SFN directly targeted p62 protein and enhanced p62/SLC7A11 protein-protein interaction, thereby promoting the lysosomal degradation of SLC7A11 and triggering ferroptosis. Notably, both subcutaneous and intratibial OS models in nude mice confirmed the ferroptosis associated anti-cancer efficacy of SFN in vivo .

2016年6月27日 · Phosphorylated p62/Sqstm1 accumulates in tumour regions positive for hepatitis C virus (HCV). An inhibitor of phosphorylated p62-dependent Nrf2 activation suppresses the proliferation and...

2017年6月15日 · Our findings show how p62 helps maintain intracellular pools of GSH needed to limit mitochondrial dysfunction in tumor cells with elevated mTORC1, highlighting p62 and redox homeostasis as nodal vulnerabilities for therapeutic targeting in these tumors.

2017年6月6日 · Metabolic profiling revealed that depletion of p62 in Tsc2-null cells decreased intracellular glutamine, glutamate, and glutathione (GSH). p62 positively regulated the glutamine transporter Slc1a5 and increased glutamine uptake in Tsc2-null cells.

2012年8月1日 · In our study, we found that HT increases p62 mRNA and protein levels dose dependently; we also found that regulation of p62 accumulation is not involved in autophagy. p62 expression and GSH content were decreased by JNK inhibitor SP600125 treatment.

p62的敲减能够改变线粒体形态，减弱线粒体膜电位及线粒体最大呼吸能力，增加线粒体活性氧自由基ros和线粒体自噬标志物pink1的表达。 功能回复实验发现，内源性谷胱甘肽表达的增加及过表达Sod2会抑制线粒体损伤、促进Tsc2无效细胞的生长。

In oral squamous cell carcinomas, p62/SQSTM1 expression is a potential marker to identify carcinogenesis, therapeutic resistance or poor prognosis, and it is a key player in glutathione (GSH) induction [5]. Defective autophagic lysosomal pathway leads to accumulation of p62/SQSTM1, which promotes oncogenesis by itself and genome instability [6].

检测细胞内铁（Fe）、还原型谷胱甘肽（GSH）、脂质过氧化物（MDA）、活性氧（ROS）的含量；Western blot检测FTH1、GPX4、xCT、SLC40A1、p62、Keapl、NRF2的表达水平，JC-1染色检测线粒体膜电位，细胞免疫荧光检测p62、Keap1表达情况。 ... </sec><sec> 结论 鳖甲煎丸可 …

SQSTM1/p62 是由SQSTM1基因编码的一种多功能泛素化结合的接头蛋白,参与泛素蛋白酶体系统和自噬-溶酶体系统两种蛋白质降解过程,同时具有质核穿梭功能,在DNA损伤修复和氧化应激反应中发挥重要作用。 p62作为细胞接头蛋白,是细胞内多种蛋白复合物的主要成分[1],其分子结构. 细胞的生长、自噬、癌变等生理病理过程。 p62不仅是自噬体与底物之间的适配蛋白,反过来也调节着自噬的过程。 当自噬活性减弱时,p62蛋白会在细胞质中累积,是反映自噬活性的标记蛋白之一[5]。 …