2022年2月22日 · We found that after LPS treatment, ablation of ATG9A caused a marked decrease in the phosphorylation of JNK but not IκB kinase (IKK) or p65, compared with the controls (Figure 7H). 3-Methyladenine (3-MA) is a widely used inhibitor of PI3KC3/VPS34 activity.

2025年1月21日 · k48和k63经常是多聚泛素化的最主要的两种形式，通过k48连接的多聚泛素化通常会导致蛋白酶体介导的蛋白降解，k63连接的多聚泛素化可以参与信号调节，对很多信号通路的表达激活有重要作用

2024年7月9日 · Deletion of PPARγ in murine embryonic fibroblasts resulted in increased stability of p65. Conversely, overexpression of PPARγ led to ubiquitination of p65 at the K48 site, resulting in a rapid decrease in p65 half-life. Studies on the K28 mutant of p65 revealed a counteractive effect on PPARγ-mediated ubiquitination and degradation.

TRIM21 interacted with IκBα by PRY/SPRY domain, stabilizes IκBα and negatively regulates IκBα phosphorylation in macrophages during C. pseudotuberculosis infection. In addition, TRIM21 positively regulates the ubiquitination of IκBα via K48 linkage rather than K63 linkage in C. pseudotuberculosis -infected macrophages.

2024年12月20日 · Low expression of TRAF6 inhibited nuclear translocation of NF-κB-p65, which was achieved by promoting the expression of Interferon regulatory factor 3 (IRF3). Importantly, TRAF6, an E3 ubiquitin ligase, bound to the IRF3-Δ (SR + IAD) (1-190aa) domain, inducing Lys70 ubiquitination of IRF3 to regulate its protein stability, with ubiquitin K48 ...

2019年8月21日 · Mechanistically, RNF182 promotes the degradation of p65 via K48-linked ubiquitination, resulting in the inhibition of TLR-triggered innate immune responses. Our findings highlight a feedback-negative mechanism for terminating TLR-induced inflammation and maintaining the immunological balance.

NF-κB p65 是NF-κB家族成员之一，NF-κB是一种多效性转录因子，几乎存在于所有细胞类型中，并参与许多生物学过程，例如炎症、免疫、分化、细胞生长、肿瘤发生和凋亡。 NF-κB受翻译后修饰和亚细胞区室化的各种机制以及与其他辅因子或共抑制因子的相互作用的控制。 p65-RelA是哺乳动物NF-κB家族的成员，该家族由5个亚基组成：p50-NF-κB1，p52-NF-κB2，c-Rel，p65-RelA和RelB。

FR promotes the K48-linked ubiquitination of p65 by recruiting the E3 ubiquitin enzyme RNF182. Tenocytes were silenced with RNF182 siRNA (A), ING4 siRNA (B), or PPARγ siRNA (C) for 24 h and...

2022年2月22日 · We find that, upon ROS stimulation, TRAF6 mediates K48/K63-linked ubiquitination of ATG9A, enhancing ATG9A-Beclin 1 interaction, PI3KC3/VPS34 activity, and autophagy activation. These results reveal a role for ATG9A ubiquitination in regulating oxidative stress-induced autophagy.

... we found that FR specifically increased K48-linked (K48-only ubiquitin mutant) poly-ubiquitination of p65, but not the ubiquitination of p65 with other ubiquitin linkages in an...