Silencing OSM inhibits ox-LDL-induced p65-NLRP3 pathway. The underlying mechanisms of OSM in the event of atherosclerosis were then detected. Western blot analysis showed that ox-LDL causes significant upregulation of NLRP3 and phosphorylated p65. Transfection of cells with OSM siRNAs repressed the activation of NLRP3 and p65, in contrast to si ...

Silencing OSM significantly inhibited ox-LDL-induced cytokine release, including TNF-α, IL-1β, IL-6, and IL-18. Ox-LDL activated p65 and NLRP3, which further induced caspase-1 cleavage, apoptosis-associated, speck-like protein containing a caspase-1 recruitment domain (ASC) upregulation, and gasdermin-D (GSDMD)-N fragmentation.

Based on the results of a chromatin immunoprecipitation (ChIP) assay, adiponectin-induced binding of p65 at the OSM promoter was attenuated by Ly294002, Wortmannin, and an Akt inhibitor (...

Our data showed that stimulation of osteoblasts with adiponectin increased NF-κB translocation and activated p65 biding to NF-κB at the OSM promoter, indicating that NF-κB is one of the most important transcription factor binding sites for adiponectin-induced OSM expression during inflammatory responses.

Stimulation of cells with CCN1 increased phosphorylation of FAK, c-Src, PI3K, and NF-κB via αvβ3 receptor; CCN1 treatment of osteoblasts increased NF-κB-luciferase activity and p65 binding to NF-κB element on OSM promoter.

NF-κB p65 是NF-κB家族成员之一，NF-κB是一种多效性转录因子，几乎存在于所有细胞类型中，并参与许多生物学过程，例如炎症、免疫、分化、细胞生长、肿瘤发生和凋亡。 NF-κB受翻译后修饰和亚细胞区室化的各种机制以及与其他辅因子或共抑制因子的相互作用的控制。 p65-RelA是哺乳动物NF-κB家族的成员，该家族由5个亚基组成：p50-NF-κB1，p52-NF-κB2，c-Rel，p65-RelA和RelB。

2024年12月25日 · p65蛋白，也被称为RelA，是NF-κB家族中的一个关键转录因子。 NF-κB家族包括P50、P52、RelA（p65）、c-Rel和RelB五个成员，它们均含有Rel样结构域，是参与多种生物学过程的多效性转录因子。

2019年3月12日 · Importantly, we confirm that OSM induces at least a 4-fold increase in IL-6 production from estrogen receptor-negative (ER-) breast cancer cells in a manner that is dependent on STAT3 signaling. Furthermore, OSM induces STAT3 phosphorylation and IL-1β promotes p65 phosphorylation to synergistically induce IL-6 secretion in ER- MDA-MB-231 and ...

Our data demonstrate that in microglia, OSM activates the NF-κB p65 pathway and promotes recruitment of P-p65 to the TNF-α and iNOS promoters . OSM stimulation also enhances recruitment of the co-activator p300, which likely associates with P-p65, and helps drive TNF-α and iNOS gene expression.

Our data demonstrate that in microglia, OSM activates the NF-κB p65 pathway and promotes recruitment of P-p65 to the TNF-α and iNOS promoters (Figure 8). OSM stimulation also enhances recruitment of the co-activator p300, which likely associates with P-p65, and helps drive TNF-α and iNOS gene expression.