More specifically the p65 subunit of NFkB binds the DBD of RXR α, which interferes with PXR-RXR α heterodimer formation and thus prevents the PXR-RXR α heterodimer from binding XREM in...

2006年6月30日 · NF-κB p65 directly interacted with the DNA-binding domain of RXRα and may prevent its binding to the consensus DNA sequences, thus inhibiting the transactivation by the PXR·RXRα complex. This mechanism of suppression by NF-κB activation may be extended to other nuclear receptor-regulated systems where RXRα is a dimerization partner.

1999年3月19日 · In this study, we found that retinoids inhibit this LPS-stimulated production of IL-12 in a dose-dependent manner. The NFκB components p50 and p65 bound retinoid X receptor (RXR) in a ligand-independent manner in vitro, and the interaction interfaces involved the p50 residues 1–245, the p65 residues 194–441, and the N-terminal A/B/C domains of RXR.

2015年3月1日 · Upon ligand activation, DNA-bound PPARα directly interacts with p65 to abolish its binding to an NFκB response element (NRE) in the complement C3 promoter. (C) PPARα directly interacts with pro-inflammatory transcription factors cJun and p65 to negatively regulate their target genes by a mechanism that is thought to be PPRE-independent.

2024年12月25日 · p65磷酸化位点对PKC介导的信号转导通路具有重要的调控作用，磷酸化后的p65蛋白在核转运、DNA结合和转录活性等方面发生显著变化，进而影响下游基因的表达。

1999年3月19日 · Lipopolysaccharide (LPS) increases the production of interleukin-12 (IL-12) from mouse macrophages via a kappaB site within the IL-12 p40 promoter. In this study, we found that retinoids inhibit this LPS-stimulated production of IL-12 in a dose-dependent manner. The NFkappaB components p50 and p65 b …

2007年4月20日 · Supershift and chromatin immunoprecipitation assays showed in vitro and in vivo binding of NF-κB subunit p65 and the retinoic acid receptors, RARα and RXRα, to the promoter sequences.

2021年1月2日 · LPS also caused an increase in expression of TLR4, MyD88, phosphorylated TAK1, NF-κB p65, phosphorylated NF-κB p65, COX-2, and IL-1β proteins in addition to COX-2 activity and levels of PGE 2 and IL-1β. The LPS-induced changes were prevented by a selective RXR agonist, bexarotene.

2005年1月1日 · The p65 RelA subunit of NF-kappaB represses MMTV expression on either transient or integrated reporters. In contrast, the viral oncoprotein E1A represses a transient but not an integrated MMTV.

LPS-stimulated NF-κB p65 dynamic response marks the initiation of TNF expression and transition to IL-10 expression in RAW 264.7 macrophages. During injury and infection, inflammation is a response by macrophages to effect healing and repair.