2 天之前 · 研究表明，PABP增强eIF4G1、eIF4A和eIF4B在翻译终止中的活性，尤其是在poly(A)尾存在时。 PABP通过直接与eIF4G1和eIF4B相互作用来增强活性，而eIF4A则可能通过与其他因子的相互作用发挥作用。尽管PABP促进了翻译终止，但并非必需因素。

聚（A）结合蛋白（PABP）促进了43S PIC对5′帽的招募，PABP通过与eIF4F复合物中的eIF4G相互作用从mRNA的3′聚（A）尾部绕回来。 在对胁迫的反应中，一般的翻译启动往往通过胁迫诱导的 eIF2α 的磷酸化而被抑制，这减少了活性三元复合物的可用性。 某些胁迫反应的mRNA，如酵母中的一般控制非抑制性4（GCN4）和哺乳动物中的激活转录因子4（ATF4），其翻译通常被其mRNA中的上游开放阅读框（uORF）所抑制，优先在三元复合物的降低水平上翻译。 然而， …

2023年1月31日 · Most mRNAs are circularized via the eIF4E–eIF4G–PABP interaction, which stabilizes mRNAs and enhances translation by recycling ribosomes. However, studies using single-molecule fluorescence...

2018年1月30日 · Despite their divergence, eIF4G and eIFiso4G interact with eIF4A, eIF4B, and eIF4E isoforms needed for binding an mRNA. eIF4G and eIFiso4G also interact with the poly(A)-binding protein (PABP) which promotes ribosome recruitment to an mRNA.

1998年4月2日 · Here, we describe a new human PABP-interacting protein, PAIP-1, whose sequence is similar to the central portion of eIF4G and which interacts with eIF4A. Overexpression of PAIP-1 in COS-7 cells...

Poly(A)-binding protein (PABP) stimulates translation initiation by binding simultaneously to the mRNA poly(A) tail and eukaryotic translation initiation factor 4G (eIF4G). PABP activity is regulated by PABP-interacting (Paip) proteins. Paip1 binds ...

2018年11月28日 · Here, we show that PABP interacts with the 40S and 60S ribosomal subunits dynamically via RRM2-3 or RRM3-4. Using a reconstituted protein expression system, we demonstrate that wild-type PABP...

The poly(A)-binding protein (PABP), a protein that contains four conserved RNA recognition motifs (RRM1–4) and a C-terminal domain, is expressed throughout the eukaryotic kingdom and promotes translation through physical and functional interactions with eukaryotic initiation factor (eIF) 4G and eIF4B.

5 天之前 · The eRF1–eRF3–GTP complex then associates with either the same or a neighboring PABP molecule on the poly (A) tail. As the elongating ribosome encounters the stop codon and stalls while waiting for eRF1, eIF4A and eIF4B from the nearby PABP–eIF4F complex bind to preTC and induce its conformational changes.

2010年9月28日 · Eukaryotic translation initiation factor 4G (eIF4G) functions to organize the assembly of initiation factors required for the recruitment of a 40S ribosomal subunit to an mRNA and for interacting with the poly(A) binding protein (PABP). Many eukaryotes express two highly similar eIF4G isoforms. eIFi …