丙酮酸彻底氧化分解需要先转变为乙酰辅酶A，由丙酮酸脱氢酶复合体（pyruvate dehydrogenase complex，PDH或PDC）催化。 这个反应需要三种酶连续催化，依次称为E1（丙酮酸脱氢酶，pyruvate dehydrogenase (acetyl-tr…

由1可知，tgf-β抑制pdk4表达，因此可能促进pdh介导的ac-coa生物合成。 敲低PDK4显著抑制PDH磷酸化并上调Ac-CoA（图2A-B）。 PDK不同剪切异构体中，PDK4显著促进EMT，PDK3对EMT有一定的促进作用，而PDK1和PDK2对EMT没有影响（图2C-D）。

Pyruvate dehydrogenase kinase (PDK) can regulate the catalytic activity of pyruvate decarboxylation oxidation via the mitochondrial pyruvate dehydrogenase complex, and it further links glycolysis with the tricarboxylic acid cycle and ATP generation.

Pyruvate dehydrogenase kinase (also pyruvate dehydrogenase complex kinase, PDC kinase, or PDK; EC 2.7.11.2) is a kinase enzyme which acts to inactivate the enzyme pyruvate dehydrogenase by phosphorylating it using ATP.

用western blot、流式细胞术或免疫细胞化学分析活细胞或细胞和组织提取物中的PDH亚基. PDH复合物缺陷是乳酸性酸中毒的重要原因，可引起儿童利氏病。大多数PDH缺乏病例是由于x -连锁e1 - α亚基的突变，尽管其他亚基的突变也有报道。

PDK inhibition by pyruvate facilitates PDH activation, favoring glucose oxidation and malonyl-CoA formation: the latter suppresses long-chain fatty acid (LCFA) oxidation. PDK activation by high mitochondrial acetyl-CoA/CoA and NADH/NAD(+) concentration ratios that reflect high rates of LCFA oxidation causes blockade of glucose oxidation [ 18 ...

Pyruvate Dehydrogenase Kinase (PDK) refers to an enzyme that regulates the activity of Pyruvate Dehydrogenase (PDH) by phosphorylating it. When PDH is phosphorylated by PDK, it becomes inactive. PDK plays a crucial role in the control of glucose metabolism and energy production in the mitochondria.

1 天前 · Additionally, they inhibit respiration by upregulating PDH kinase 1 (PDK1) 36,37, an enzyme that phosphorylates and inactivates PDH, reducing the conversion of pyruvate to acetyl-CoA 38 and its ...

Pyruvate dehydrogenase kinase (PDK) 1 is one of four isozymes that inhibit the oxidative decarboxylation of pyruvate to acetyl-CoA via pyruvate dehydrogenase. PDK activity is elevated in fasting or starvation conditions to conserve carbohydrate reserves. PDK has also been shown to increase mitochondrial fatty acid utilization. In cardiomyocytes, metabolic flexibility is …

2024年3月1日 · Compound g25 was discovered to target the pyruvate dehydrogenase kinase (PDK)/pyruvate dehydrogenase (PDH) axis and activate PDH. g25 improved obesity and blood lipid levels in a diet-induced obesity mouse model, while also enhancing glucose tolerance in …